The impact of different Lactobacillus casei doses on the intestinal microbiota in mice was evaluated in this experiment. Seventy-two mice weighing (20±2) g were selected and randomly divided into six groups: normal control group, model control group, positive drug control group, L. casei low-dose group, medium-dose group and high-dose group, respectively. Mice were fed with compound diphenoxylate to establish a constipation model. Mice in the normal control group were fed with saline, and mice in the model control group were fed with compound difenoxil and saline, meanwhile, mice in the positive control group were fed with compound difenoxil and Streptococcus thermophilus, and mice in the remaining groups with constipation were fed with compound difenoxil and corresponding concentrations of L. casei. After 3 days of gavage, feces were collected from the intestinal tract of the mice for analysis of flora composition. The results showed that the abundance of Lactobacillus and Alistipes in the intestinal flora of constipated mice was reduced to different degrees, while the abundance of Prevotella and norank_f_Muribaculaceae was increased compared with the normal control group. After 3 days of Lactobacillus casei intervention, the dominant flora were norank_f_Muribaculaceae, Lactobacillus, Prevotella and Cryobacillus. Compared with the model control group, the abundance of Prevotella and norank_f_Muribaculaceae in the intestinal flora of mice in L. casei groups decreased, and the abundance of Alistipes increased; the abundance of Lactobacillus in the L. casei medium-dose group and high-dose group increased. It is suggested that L. casei can improve the intestinal flora and relieve constipation by inhibiting the growth of Prevotella and promoting the growth of Lactobacillus and Alistipes in the intestinal tract.
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