Changes of soybean isoflavones and flavor substances in fermented soybean milk

  • LIU Ting ,
  • ZHAO Liangzhong ,
  • ZHOU Xiaohu ,
  • HE Chuman ,
  • ZHANG Yanyan ,
  • ZHANG Xuejiao
Expand
  • 1(College of Food Science and Chemical Engineering, Shaoyang University, Shaoyang 422300, China)
    2(Hunan Provincial Key Laboratory of Soybean Products Processing and Safety Control, Shaoyang 422300, China)
    3(Shaoyang Food and Drug Inspection Institute, Shaoyang 422300, China)

Received date: 2021-10-21

  Revised date: 2021-11-19

  Online published: 2022-04-25

Abstract

To investigate the substrate utilization and changes of soybean isoflavone metabolism and flavor substances during the fermentation of soybean milk, different kinds and concentrations of carbon sources were added to soybean milk. Acidity and sensory score were taken as evaluation indexes. The results showed that with the increase of sucrose, lactose and glucose ratio, the sensory scores of taste, smell and comprehensive acceptance improved significantly (P<0.05), while the sensory scores of texture and color were not affected. The contents of isoflavones glycoside decreased linearly, and fermented soybean milk with 0.03 g/mL gluconate had the highest decreasing rates of isoflavones daidzein and genitin, up to 92.62% and 88.13%, respectively. The aglycone isoflavones of fermented soybean milk with 0.03 g/mL sucrose were transformed firstly. During the fermentation of soybean milk, a total of 72 volatile compounds were detected by GC-MS, including seventeen esters, sixteen acids, fifteen ketone, eleven alcohols, four aldehydes. The substances with unpleasant flavor, such as 1-octene-3-ol and hexanal, could not be detected after fermentation, and new pleasant flavors were formed.

Cite this article

LIU Ting , ZHAO Liangzhong , ZHOU Xiaohu , HE Chuman , ZHANG Yanyan , ZHANG Xuejiao . Changes of soybean isoflavones and flavor substances in fermented soybean milk[J]. Food and Fermentation Industries, 2022 , 48(6) : 181 -187 . DOI: 10.13995/j.cnki.11-1802/ts.029778

References

[1] 刘婷, 赵良忠, 李明, 等.乳酸菌发酵酸豆奶中不良风味物质研究进展[J].食品安全质量检测学报, 2021, 12(14):5 720-5 726.
LIU T, ZHAO L Z, LI M, et al.Research progress on off-flavor substancein fermented soybean milk by lactic acid bacteria[J].J Journal of Food Safety & Quality, 2021,12(14):5 720-5 726.
[2] 陈倩. 大豆酸乳发酵菌种以及工艺条件优化的研究[D].杭州:浙江工商大学, 2015.
CHEN Q.The study of the strains and the fermentation process optimization of soy yogurt[D].Hangzhou:Zhejiang Technology and Business University, 2015.
[3] 施小迪. 豆乳及豆乳发酵液不良风味的形成及改善途径研究[D].北京:中国农业大学, 2017.
SHI X D.Study on the formation and improving methods for off-flavor of soymilk and soymilk fermented liquid[D].Beijing:China Agricultural University, 2017.
[4] 周艳平, 张彩猛, 孔祥珍, 等.酸奶发酵剂对大豆酸奶品质的影响[J].大豆科学, 2018, 37(1):149-156.
ZHOU Y P, ZHANG C M, KONG X Z, et al.Effect of yoghurt starter cultures on the quality of soybean yoghurt[J].Soybean Science, 2018, 37(1):149-156.
[5] 武旭. 利用纳豆芽孢杆菌与乳酸菌发酵全豆豆乳的研究[D].无锡:江南大学, 2016.
WU X.Study on fermentation of whole soybean milk by Bacillussubtilis natto and Lactobacillus[D].Wuxi:Jiangnan University, 2016.
[6] 郭帅. 适用于豆乳发酵的嗜热链球菌筛选及其应用研究[D].呼和浩特:内蒙古农业大学, 2019.
GUO S.Screening and application of Streptococcus thermophilus suitable for soymilk fermentation[D].Hohhot:Inner Mongolia Agricultural University, 2019.
[7] 李程程. 产黏植物乳杆菌 70810 发酵豆乳生产拉丝酸豆乳饮品的研制[D].南京:南京农业大学, 2010.
LI C C.Production of drawing soy beverage produced with sticky Lactobacilius plantarum 70810[D].Nanjing:Nanjing Agricultural University, 2010.
[8] 欧红艳, 赵良忠, 李明, 等.豆清饮料发酵过程中大豆异黄酮及风味物质变化规律[J].食品与发酵工业,2021,47(24):95-101.
OU H Y, ZHAO L Z, LI M, et al.Changes of soybean isoflavones and flavor substances in soybean whey beverage during fermentation[J].Food and Fermentation Industries,2021,47(24):95-101.
[9] 尹乐斌, 杨莹, 陈浩, 等.固相微萃取-气质联用法测定湘派豆干老卤挥发性风味物质变化[J].中国调味品, 2021, 46(8):147-154.
YIN L B, YANG Y, CHEN H, et al.Determination of volative flavor compounds in xiangpai dried tofu brineby solid-phase microextraction and mass spectrometry[J].China Condiment, 2021, 46(8):147-154.
[10] 王浩. 牛奶组分对益生菌酸豆乳的影响研究[D].广州:华南理工大学, 2011.
WANG H.Studies on the effect of milk compositions on the probiotics soy yogurt[D].Guangzhou:South China University of Technology, 2011.
[11] 张秋香, 应聪萍, 刘思思, 等.乳酸菌利用低聚果糖和低聚木糖的特性研究[J].食品与生物技术学报, 2020, 39(4):18-23.
ZHANG Q X, YING C P, LIU S S, et al.Fructooligosaccharides and xylooligosaccharides utilization properties of lactic acid bacteria[J].Journal of Food Science and Biotechnology, 2020, 39(4):18-23.
[12] 陈卫. 乳酸菌科学与技术[M].北京:科学出版社, 2018.
CHEN W.Science and Technology of Lactic Acid Bacteria[M].Beijing:Science Press, 2018.
[13] FEI Y T, LIU L, LIU D M, et al.Investigation on the safety of Lactobacillus amylolyticus L6 and its fermentation properties of tofu whey[J].LWT - Food Science and Technology, 2017, 84:314-322.
[14] 陈嘉序, 陈如扬, 连媛, 等.大豆异黄酮的生物转化及功能活性研究进展[J].食品研究与开发, 2021, 42(9):176-182.
CHEN J X, CHEN R Y, LIAN Y, et al.Progress in microbial conversion and functional activity of soy isoflavones[J].Food Research and Development, 2021, 42(9):176-182.
[15] 周帆. 高转化大豆异黄酮乳酸菌的筛选及在发酵豆乳中的应用[D].扬州:扬州大学, 2021.
ZHOU F.Screening of lactic acid bacteria with hign conversion of soy isoflavones and application in fermented soymilk[D].Yangzhou:Yangzhou University, 2021.
[16] TSANGLIS D, ASHTON J, MCGILL A, et al.Biotransformation of isoflavones by bifidobacteria in fermented soymilk supplemented with D-glucose and L-cysteine[J].Journal of Food Science, 2010, 68(2):623-631.
[17] CHEN K I, ERH M H, SU N W, et al.Soyfoods and soybean products:From traditional use to modern appiications[J].Applied Microbiology and Biotechnology, 2012, 96(1):9-22.
[18] 朱亚军.豆酸奶适制性菌株的筛选及发酵剂的研制[D].广州:华南农业大学, 2018.
ZHU Y J.Screening of suitable strains for fermented soymilk and development of starter [D].Guangzhou:South China Agricultural University, 2018.
[19] CHENG H.Volatile flavor compounds in yogurt:A review[J].Critical Reviews in Food Science & Nutrition, 2010, 50(10):938-950.
[20] 薛瑞琪, 夏小乐, 褐色发酵乳中5-羟甲基糠醛相关性分析及应用[J].食品发酵与工业, 2021, 47(19):79-83.
XUE R Q, XIA X L.Correlation analysis of 5-hydroxymethylfurfural in brown fermented milk[J].Food and Fermentation Industries, 2021, 47(19):79-83.
[21] 王昕悦, 曹少军, 赵华杰, 等.不同菌种发酵苹果浓缩汁的风味成分变化分析[J].食品与发酵工业, 2017, 43(4):238-243.
WANG X Y, CAO S J, ZHAO H J, et al.Analysis on flavor compounds of concentrated apple juice fermented by different strains[J].Food and Fermentation Industries, 2017, 43(4):238-243.
Outlines

/