Hypoxanthine conversion yields, cell growth and cyclic adenosine monophosphate (cAMP) production are lower when cAMP is produced via salvage pathway using hypoxanthine as the precursor substance. To solve these problems, shaking flask fermentation was carried out by feeding quercetin and luteolin during the fermentation. The results showed that the yield of cAMP greatly increased. In 7 L fermenter, the conversion rates of hypoxanthine and the fermentation performance were further improved, the cAMP concentration was 5.33 g/L with an increment of 33.92% by adding 12 mg/L-broth quercetin. The results showed that xanthine oxidase activities and hypoxanthine decomposition were greatly reduced by quercetin, thereby leading to higher hypoxanthine conversion yields.
TAN Hai
,
YUAN Linna
,
LU Nanxun
,
ZHANG Zhonghua
,
CHANG Jingling
,
LI Zhigang
. High-efficient cAMP biosynthesis by inhibiting xanthine oxidase activity in Arthrobacter sp. CCTCC 2013431 with quercetin[J]. Food and Fermentation Industries, 2022
, 48(7)
: 22
-28
.
DOI: 10.13995/j.cnki.11-1802/ts.028935
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