Food and Fermentation Industries >

2022 , Vol. 48 >Issue 7: 201 - 210

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027632

Effect of fluorescence irradiation and relative humidity on the glucosinolate metabolism and contents of bioactive substance in fresh-cut broccoli

  • ZHAO Huanhuan ,
  • LUO Shufen ,
  • ZHOU Hongsheng ,
  • HU Huali ,
  • ZHANG Yingtong ,
  • LI Pengxia
Expand
  • 1(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
    2(Institute of Facilities and Equipment in Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
    3(Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China)

Received date: 2021-04-07

  Revised date: 2021-05-19

  Online published: 2022-04-27

Fold

Abstract

The aim of this study was to explore the condition of fluorescence irradiation and relative humidity (RH) that were beneficial for promoting the bioactive substance content of fresh-cut broccoli. Firstly, the effect of different conditions of fluorescence irradiation [optical density: 5-8, 25-35, 75-85 μmol/(m2·s)] along with different RH (50%-60%, 70%-80%, 90%-100%) on the phenotype, sensory score, color, and the contents of total glucosinolate and isothiocyanate (ITC) of fresh-cut broccoli were investigated. By analyzing the results of these indexes, the condition of fluorescence irradiation with an optical density at 25-35 μmol/(m2·s) and RH at 70%-80% were selected for the following experiment. Secondly, the fresh-cut broccoli was stored under the selected environment, and the actual condition of supermarket shelf [optical density: 5-8 μmol/(m2·s), RH: 50%-60%] was used as control. The effects of fluorescence irradiation and RH on the glucosinolate metabolism and contents of bioactive substance in fresh-cut broccoli were studied. The results showed that the treatment of fluorescence irradiation with an optical density at 25-35 μmol/(m2·s) together with RH at 70%-80% significantly enhanced the main glucosinolates, myrosinase activity and ITC. What's more, the sample in this selected condition of fluorescence irradiation and RH displayed higher contents of total phenols, anthocyanin, chlorophyll and carotenoid than those of control. Therefore, the suitable condition of fluorescence irradiation [optical density: 25-35 μmol/(m2·s)] together with RH (70%-80%) could maintain the quality and bioactive substance of fresh-cut broccoli, which can provide theoretical and technical support for the storage and sale of broccoli.

Key words： broccoli; fluorescence irradiation; relative humidity; glucosinolate; bioactive substance

Cite this article

ZHAO Huanhuan , LUO Shufen , ZHOU Hongsheng , HU Huali , ZHANG Yingtong , LI Pengxia . Effect of fluorescence irradiation and relative humidity on the glucosinolate metabolism and contents of bioactive substance in fresh-cut broccoli[J]. Food and Fermentation Industries, 2022 , 48(7) : 201 -210 . DOI: 10.13995/j.cnki.11-1802/ts.027632

References

[1] 蒋金勇,唐海兵,栾东磊,等.聚乳酸/聚丁二酸-己二酸丁二酯可降解抗氧化薄膜对鲜切西兰花的保鲜效果[J].食品科学,2019,40(13):274-280.
JIANG J Y,TANG H B,LUAN D L,et al.Effect of polylactic acid/poly(butylene succinate-co-adipate) biodegradable film containing different essential oils in preserving quality and extending shelf-life of fresh-cut broccoli[J].Food Science,2019,40(13):274-280.
[2] 刘泽松,史君彦,左进华,等.UV-C和LED红光复合处理对西兰花贮藏品质的影响[J].食品科学,2020,41(17):238-245.
LIU Z S,SHI J Y,ZUO J H,et al.Effect of combined UV-C and red light emitting diode irradiation on storage quality of broccoli[J].Food Science,2020,41(17):238-245.
[3] BÁRCENA A,BAHIMA J V,CASAJU′S V,et al.The degradation of chloroplast components during postharvest senescence of broccoli florets is delayed by low-intensity visible light pulses[J].Postharvest Biology and Technology,2020,168:111249.
[4] ILIĆ Z S,FALLIK E.Light quality manipulation improves vegetable quality at harvest and postharvest:A review[J].Environmental and Experimental Botany,2017,139:79-90.
[5] 詹丽娟,魏国强,乔明武,等.光照处理提高鲜切西兰花贮藏品质[J].食品科学,2012,33(14):296-300.
ZHAN L J,WEI G Q,QIAO M W,et al.Quality improvement of fresh-cut broccoli(Brassica Oleracea L.) during storage by light illumination[J].Food Science,2012,33(14):296-300.
[6] ZHANG X Y,BIAN Z H,YUAN X X,et al.A review on the effects of light-emitting diode(LED) light on the nutrients of sprouts and microgreens[J].Trends in Food Science & Technology,2020,99:203-216.
[7] JIN P,YAO D,XU F,et al.Effect of light on quality and bioactive compounds in postharvest broccoli florets[J].Food Chemistry,2015,172:705-709.
[8] JIANG A L,ZUO J H,ZHENG Q L,et al.Red LED irradiation maintains the postharvest quality of broccoli by elevating antioxidant enzyme activity and reducing the expression of senescence-related genes[J].Scientia Horticulturae,2019,251:73-79.
[9] 张国强,乔勇进,王晓,等.相对湿度对双孢蘑菇贮藏品质的影响[J].食品工业科技,2019,40(13):226-230.
ZHANG G Q,QIAO Y J,WANG X,et al.Effect of relative humidity on storage quality of Agaricus bisporus[J].Science and Technology of Food Industry,2019,40(13):226-230.
[10] KTENIOUDAKI A,O'DONNELL C P,DO NASCIMENTO NUNES M C.Modelling the biochemical and sensory changes of strawberries during storage under diverse relative humidity conditions[J].Postharvest Biology and Technology,2019,154:148-158.
[11] CALEB O J,ILTE K,FRÖHLING A,et al.Integrated modified atmosphere and humidity package design for minimally processed Broccoli(Brassica oleracea L.var.italica)[J].Postharvest Biology and Technology,2016,121:87-100.
[12] 徐长妍,刘杰,陈焕霓.鲜切西兰花的PE薄膜保鲜包装技术研究[J].食品科技,2011,36(7):40-44.
XU C Y,LIU J,CHEN H N.PE film fresh-keeping package for fresh-cut broccoli[J].Food Science and Technology,2011,36(7):40-44.
[13] 彭光华,赵安娜,涂贻轩,等.不同温度、保湿时间对西兰花主要采后病害发生的影响[J].长江蔬菜,2019(14):72-76.
PENG G H,ZHAO A N,TU Y X,et al.Effects of temperature and moisturizing time on occurrence of main post-harvest diseases of Broccoli[J].Journal of Changjiang Vegetables,2019(14):72-76.
[14] 朱玲玲,胡花丽,罗淑芬,等.褪黑素调控呼吸代谢及抗氧化活性延缓采后青花菜衰老[J].农业工程学报,2018,34(3):300-308.
ZHU L L,HU H L,LUO S F,et al.Melatonin delaying senescence of postharvest broccoli by regulating respiratory metabolism and antioxidant activity[J].Transactions of the Chinese Society of Agricultural Engineering,2018,34(3):300-308.
[15] 纪淑娟,熊振华,程顺昌,等.1-MCP和CO2自发释放处理对西兰花常温货架期的保鲜作用[J].食品与发酵工业,2014,40(2):202-206.
JI S J,XIONG Z H,CHENG S C,et al.Effects of spontaneous release 1-MCP and CO2 on the quality of broccolis during shelf life[J].Food and Fermentation Industries,2014,40(2):202-206.
[16] HEANEY R K,SPINKS E A,FENWICK G R.Improved method for the determination of the total glucosinolate content of rapeseed by determination of enzymically released glucose[J].The Analyst,1988,113(10):1 515.
[17] KIM H J,CHEN F,WANG X,et al.Effect of methyl jasmonate on phenolics,isothiocyanate,and metabolic enzymes in radish sprout(Raphanus sativus L.)[J].Journal of Agricultural and Food Chemistry,2006,54(19):7 263-7 269.
[18] YI G,LIM S,CHAE W B,et al.Root glucosinolate profiles for screening of radish(Raphanus sativus L.) genetic resources[J].Journal of Agricultural and Food Chemistry,2016,64(1):61-70.
[19] YANG R Q,HUI Q R,GU Z X,et al.Effects of CaCl2 on the metabolism of glucosinolates and the formation of isothiocyanates as well as the antioxidant capacity of broccoli sprouts[J].Journal of Functional Foods,2016,24:156-163.
[20] FERNÁNDEZ-LEÓN M F,FERNÁNDEZ-LEÓN A M,LOZANO M,et al.Altered commercial controlled atmosphere storage conditions for ‘Parhenon' broccoli plants(Brassica oleracea L.var. italica) .Influence on the outer quality parameters and on the health-promoting compounds[J].LWT-Food Science and Technology,2013,50(2):665-672.
[21] WANG Y C,LIU W J,JIANG H Y,et al.The R2R3-MYB transcription factor MdMYB24-like is involved in methyl jasmonate-induced anthocyanin biosynthesis in apple[J].Plant Physiology and Biochemistry,2019,139:273-282.
[22] 李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社,2000.
LI H S.Principles and Techniques of Plant Physiological and Biochemical Experiments[M].Beijing:Higher Education Press,2000.
[23] MARISIDDAIAH R,BASKARAN V.Bioefficacy of β-carotene is improved in rats after solubilized as equimolar dose of β-carotene and lutein in phospholipid-mixed micelles[J].Nutrition Research,2009,29(8):588-595.
[24] 李大婧,王闯,徐爱琴,等.高效液相色谱法测定叶黄素顺、反异构体[J].食品科学,2012,33(22):186-190.
LI D J,WANG C,XU A Q,et al.Determination of lutein stereoisomers by HPLC[J].Food Science,2012,33(22):186-190.
[25] 张翠翠,马亚丹,李林杰,等.可溶性糖介导的荧光照射延缓鲜切西兰花衰老黄化[J].食品科学,2019,40(9):207-212.
ZHANG C C,MA Y D,LI L J,et al.Soluble sugar-mediated yellowing delay of fresh-cut broccoli florets(Brassica oleracea var.italica) exposed to fluorescence irradiation[J].Food science,2019,40(9):207-212.
[26] PINTOS F M,HASPERUÉ J H,VICENTE A R,et al.Role of white light intensity and photoperiod during retail in broccoli shelf life[J].Postharvest Biology and Technology,2020,163:111121.
[27] RYBARCZYK-PLONSKA A,HAGEN S F,BORGE G I A,et al.Glucosinolates in broccoli(Brassica oleracea L.var.italica) as affected by postharvest temperature and radiation treatments[J].Postharvest Biology and Technology,2016,116:16-25.
[28] 薛冲,李胜,马绍英,等.不同光质对西兰花愈伤组织及萝卜硫素含量的影响[J].甘肃农业大学学报,2010,45(4):95-99.
XUE C,LI S,MA S Y,et al.Effects of different light qualities on the callus and its sulforaphen content from broccoli[J].Journal of Gansu Agricultural University,2010,45(4):95-99.
[29] 罗枫.BoPIF4和BobHLH66转录因子对采后西兰花黄化关键基因的转录调控机理研究[D].沈阳:沈阳农业大学,2019.
LUO F.Research on the transcriptional regulation mechanism of BoPIF4 and BobHLH66 transcription factors on key genes of postharvest broccoli yellowing[D].Shenyang:Shenyang Agricultural University,2019.
[30] HASPERUÉ J H,GUARDIANELLI L,RODONI L M,et al.Continuous white-blue LED light exposition delays postharvest senescence of broccoli[J].LWT-Food Science and Technology,2016,65(1):495-502.
[31] LOI M,LIUZZI V C,FANELLI F,et al.Effect of different light-emitting diode(LED) irradiation on the shelf life and phytonutrient content of broccoli(Brassica oleracea L.var.italica)[J].Food Chemistry,2019,283:206-214.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.