The ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was used to determine the isometamidium residues in milk, and the mathematic model based on this method was built for the evaluation of its uncertainty. The sample was firstly dissolved in acetonitrile solution, purified by C18 column, and then determined by ultra performance liquid chromatography-tandem mass spectrometry. Finally, was quantified by external standard method. The evaluation results showed that the uncertainty components had been mainly impacted by the introduction of the standard curve and recovery experiment. The expanded uncertainty was 0.007 8 mg/kg (k=2) when the content of phenanthridine in milk was 0.017 8 mg/kg.
ZHANG Yanfang
,
LYU Yaolong
,
LI Zhao
,
BAI Rongguo
. Evaluation of uncertainty for determining isometamidium residue in milk by UPLC-MS/MS[J]. Food and Fermentation Industries, 2022
, 48(7)
: 264
-268
.
DOI: 10.13995/j.cnki.11-1802/ts.027683
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