Homology modeling and prediction of the linear B-cell antigenic epitopes of Glycinin A1a subunits

FU Yang; XI Jun; CHEN Huibin; CHEN Yang

doi:10.13995/j.cnki.11-1802/ts.028505

Food and Fermentation Industries >

2022 , Vol. 48 >Issue 8: 22 - 27

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028505

Homology modeling and prediction of the linear B-cell antigenic epitopes of Glycinin A1a subunits

FU Yang ,
XI Jun ,
CHEN Huibin ,
CHEN Yang

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(College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China)

Received date: 2020-07-07

Revised date: 2021-08-18

Online published: 2022-05-18

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Abstract

To explore the linear B-cell epitopes of Glycinin A1a subunits, the amino acid sequence of Glycinin A1a was indexed in National Center of Biotechnology Information(NCBI), then the primary and secondary structure of Glycinin A1a were analyzed by a series of bioinformatics software. The predicted linear B-cell epitopes are ²⁴EQPQQN²⁹, ⁴⁰KPDNRI⁴⁵, ⁵⁶NPNNK⁶⁰, ⁸⁰RRPSYTNG⁸⁷, ¹¹⁴PQQPQQRGQS¹²³, ¹⁹⁷QEQGGHQSQKGKHQQEEENE²¹⁶, ²⁴⁷NEGED²⁵¹, ²⁶⁷PPTDEQQQRP²⁷⁶, ²⁸⁵DEKPQCKGK²⁹³and ²⁹⁹RPRGS³⁰³. In addition, the three-dimensional model of Glycinin A1a was built by SWISS-MODEL, and the DiscoTope 2.0 Server was used to make a fractal epitope prediction of Glycinin A1a subunit. This prediction narrows down the scope of soybean allergenic epitopes identification, and it provides a reference for the detection of allergens.

Key words： A1a subunits of Globulin G1; homology modeling; linear epitopes; conformational epitopes; bioinformation

Cite this article

FU Yang , XI Jun , CHEN Huibin , CHEN Yang . Homology modeling and prediction of the linear B-cell antigenic epitopes of Glycinin A1a subunits[J]. Food and Fermentation Industries, 2022 , 48(8) : 22 -27 . DOI: 10.13995/j.cnki.11-1802/ts.028505

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