The loop-mediated isothermal amplification (LAMP) assay for the detection of Katsuwonus pelamis was developed by using four primers including two inner primers and two outer primers that specifically amplify segments of the Cyt b gene. The optimized amplification conditions were determined as follows: Inner primers (FIP and BIP), 1.6 μmol/L; outer primers (F3 and B3), 0.2 μmol/L; MgSO4, 6 mmol/L; dNTP Mix, 1.6 mmol/L. The detection limit of the LAMP method was 50 pg/μL. Fifty-nine commercially samples were detected by using this LAMP method, in which 6 were positive, which consist with the DNA barcode detection result. Therefore, the LAMP detection method established in this study could be applied to the rapid and specific detection of bonito components in tuna products and provide technical support for regulatory agencies.
XU Wenjie
,
LI Qiuping
,
CUI Xiaowen
,
CAO Min
,
LI Yi
,
XIONG Xiaohui
,
XIONG Xiong
. Development of a loop-mediated isothermal amplification method for the rapid detection of Katsuwonus pelamis in tuna products[J]. Food and Fermentation Industries, 2022
, 48(8)
: 233
-238
.
DOI: 10.13995/j.cnki.11-1802/ts.028367
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