A simple and fast method for the determination of nicotinamide mononucleotide (NMN) in queen bee larva by ultra-high performance liquid chromatographic tandem mass spectrometry (UHPLC-MS/MS) was developed in this study. The NMN was separated on Waters X Select HSS T3 column (2.1 mm×100 mm,2.5 μm) and quantified on multiple reaction monitor (MRM). The method was validated using spiked samples with NMN standard of 20, 40 and 80 μg/g. Recoveries were 109.9%-111.2% with relative standard deviation (RSD) 0.87%-3.31%. The calibration curve was fitted with the correlation coefficient R2 = 0.999 7 in the range of 50-1 000 ng/mL. The limits of detection (LOD) and the limits of quantitation (LOQ) were 11.2 and 37.4 μg/kg, respectively. The proposed UHPLC-MS/MS method was applied to the analysis of queen bee larva samples. The results showed that the NMN in 11 fresh queen bee larva samples was from 37.0 to 65.8 μg/g, especially in 1 freeze-dried powder sample which was 78.0 μg/g. The NMN in queen bee larva was easily broken down, and the degradation rates at room temperature and 4 ℃ for 7 d were 96.5% and 88.5%, respectively. Low temperature storage or lyophilization could prevent the degradation of NMN in queen bee larva. As a functional and nutritional component, NMN can be used as a potential indicator for the quality evaluation of queen bee larva and their products.
ZHAO Tong
,
ZHAO Hongmu
,
SONG Meijie
,
DU Wei
,
WU Liming
,
FAN Fangfang
,
XUE Xiaofeng
. Determination of nicotinamide mononucleotide in queen bee larva by ultra-high performance liquid chromatographic tandem mass spectrometry[J]. Food and Fermentation Industries, 2022
, 48(8)
: 265
-269
.
DOI: 10.13995/j.cnki.11-1802/ts.028113
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