Food and Fermentation Industries >

2022 , Vol. 48 >Issue 8: 320 - 328

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028198

Progress on the development of immunoassays for pyrethroids insecticides and their metabolites

  • XU Zihong ,
  • LUO Lin ,
  • CHEN Zijian ,
  • WANG Bingzhi ,
  • YAN Yiyong ,
  • SUN Yuanming ,
  • XU Zhenlin
Expand
  • 1(Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China)
    2(Bioeasy Technology, Inc., Shenzhen 518000, China)

Received date: 2021-06-04

  Revised date: 2021-07-20

  Online published: 2022-05-18

Fold

Abstract

Pyrethroid pesticides are a class of nerve agents that are widely used to control pests in the world. Due to the extensive usage and toxicity, it is essential to develop screening methods suitable for rapid detection in the field of the protection of physical and mental health of consumers. Immunoassay based on the specific interaction of antibody and antigen is characterized as simplicity, sensitivity, rapidity and low cost. It has been established as the main rapid method for food safety at present. This review focused on the research progress of immunoassay for pyrethroid pesticides and their metabolites, included the hapten design strategies, antibody preparation, as well as the development of novel immunoassay methods. The application status, existing problems and prospects were also briefly discussed.

Key words： pyrethroids; immunoassay; hapten design; antibody preparation

Cite this article

XU Zihong , LUO Lin , CHEN Zijian , WANG Bingzhi , YAN Yiyong , SUN Yuanming , XU Zhenlin . Progress on the development of immunoassays for pyrethroids insecticides and their metabolites[J]. Food and Fermentation Industries, 2022 , 48(8) : 320 -328 . DOI: 10.13995/j.cnki.11-1802/ts.028198

References

[1] CHRUSTEK A, HOYN′SKA-IWAN I, DZIEMBOWSKA I, et al.Current research on the safety of pyrethroids used as insecticides[J].Medicina, 2018, 54(4):61.
[2] COSTA L G.The neurotoxicity of organochlorine and pyrethroid pesticides[J].Handbook of Clinical Neurology, 2015, 131:135-148.
[3] SODERLUND D M, CLARK J M, SHEETS L P, et al.Mechanisms of pyrethroid neurotoxicity:Implications for cumulative risk assessment[J].Toxicology, 2002, 171(1):3-59.
[4] RAY D E, FRY J R.A reassessment of the neurotoxicity of pyrethroid insecticides[J].Pharmacology & Therapeutics, 2006, 111(1):174-193.
[5] KIM H J, AHN K C, MA S J, et al.Development of sensitive immunoassays for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative human urinary biomarker for pyrethroid exposure[J].Journal of Agricultural and Food Chemistry, 2007, 55(10):3 750-3 757.
[6] 唐宇梅, 曾运婷.拟除虫菊酯:毒性和健康风险[J].中国科技信息, 2019(S1):111-112.
TANG Y M, ZENG Y T.Toxicity and health risks of pyrethroids[J].Science and Technology Information, 2019(S1):111-112.
[7] 刘凤海, 荣胜忠, 牛莹莹, 等.拟除虫菊酯类农药残留检测的研究进展[J].环境与健康杂志, 2018, 35(8):741-744.
LIU F H, RONG S Z, NIU Y Y, et al. Determination of synthetic pyrethriods pesticide residue: A review of recent studies[J]. Journal of Environment and Health, 2018, 35(8):741-744.
[8] 邓维琴, 刘书亮, 姚开.微生物降解3-苯氧基苯甲酸的研究进展[J].微生物学报, 2015, 55(9):1 081-1 088.
DENG W Q, LIU S L, YAO K. Microbial degradation of 3-phenoxybenzoic acid-A review[J]. Acta Microbiologica Sinica, 2015, 55(9):1 081-1 088.
[9] 杜付然. 气相色谱法测定地表水中拟除虫菊酯类农药残留[J].化学分析计量, 2020, 29(1):71-74.
DU F R.Determination of pyrethroid pesticide residues in surface water by gas chromatography[J].Chemical Analysis and Meterage, 2020, 29(1):71-74.
[10] 于玲, 董丽丽.分散固相萃取-超声辅助分散液液微萃取/高效液相色谱法测定土壤中溴氰菊酯残留[J].分析科学学报, 2016, 32(2):218-222.
YU L, DONG L L.Determination of deltamethrin residues in soil by dispersive solid phase extraction and ultrasound-assisted dispersive liquid-liquid microextraction coupled with high performance liquid chromatography[J].Chinese Journal of Analytical Science, 2016, 32(2):218-222.
[11] MOLONEY M, TUCK S, RAMKUMAR A, et al.Determination of pyrethrin and pyrethroid residues in animal fat using liquid chromatography coupled to tandem mass spectrometry[J].Journal of Chromatography B, 2018,1 077-1 078:60-70.
[12] 唐建设, 张敏, 陆贻通.拟除虫菊酯类农药免疫分析人工抗原研究进展[J].科技通报, 2007,23(6):835-841.
TANG J S, ZHANG M, LU Y T. The progress of synthesis methods of pyrethroids immunogene[J]. Bulletin of Science and Technology, 2007, 23(6):835-841.
[13] HUA X D, LIU X F, YIN W, et al.A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the detection of bifenthrin in a chemical soil barrier[J].Science of The Total Environment, 2015,502:246-251.
[14] 张献忠. 溴氰菊酯农药残留免疫检测技术研究[D].北京:中国农业科学院, 2008.
ZHANG X Z.Research on the immunoassay technology for the deltamethrin residue[D].Beijing:Chinese Academy of Agricultural Sciences, 2008.
[15] SONG Y, LU Y, LIU B, et al.A sensitivity-improved enzyme-linked immunosorbent assay for fenvalerate:A new approach for hapten synthesis and application to tea samples[J].Journal of the Science of Food and Agriculture,2011, 91(12):2 210-2 216.
[16] LU Y, XU N, ZHANG Y, et al.Development of general immunoassays for pyrethroids:A new approach for hapten synthesis using pyrethroid metabolite analogue and application to food samples[J].Food and Agricultural, 2010, 21(1):27-45.
[17] ZHANG Q, ZHANG W, WANG X P, et al.Immunoassay development for the class-specific assay for types I and Ⅱ pyrethroid insecticides in water samples[J].Molecules, 2010,15(1):164-177.
[18] 吴元元, 金朵, 付骋宇, 等.拟除虫菊酯类农药单链可变区抗体的制备、鉴定及其特异性分析[J].食品安全质量检测学报, 2017, 8(2):386-393.
WU Y Y, JIN D, FU C Y, et al. Preparation, characterization and specificity analysis of single-chain fragment variable antibody against pyrethroid pesticides[J]. Journal of Food Safety & Quality, 2017, 8(2):386-393.
[19] WANG J P, YU G C, SHENG W, et al.Development of an enzyme-linked immunosorbent assay based a monoclonal antibody for the detection of pyrethroids with phenoxybenzene multiresidue in river water[J].Journal of Agricultural & Food Chemistry, 2011, 59(7):2 997-3 003.
[20] KIM H J, MCCOY M R, MAJKOVA Z, et al.Isolation of alpaca anti-hapten heavy chain single domain antibodies for development of sensitive immunoassay[J].Analytical Chemistry, 2012, 84(2):1 165-1 171.
[21] ZHAO Y Y, LIANG Y, LIU Y, et al.Isolation of broad-specificity domain antibody from phage library for development of pyrethroid immunoassay[J].Analytical Biochemistry, 2016, 502:1-7.
[22] WANG Y L, LI Z F, BARNYCH B, et al. Investigation of the small size of nanobodies for a sensitive fluorescence polarization immunoassay for small molecules: 3-phenoxybenzoic acid, an exposure biomarker of pyrethroid insecticides as a model[J]. Journal of Agricultural and Food Chemistry, 2019, 67(41):11 536-11 541.
[23] 常继辰. 拟除虫菊酯噬菌体抗体库构建及基因工程抗体制备[D].天津:天津科技大学, 2014.
CHANG J C.Construction of phage antibody library specific for pyrethroids and preparation of genetically engineered antibody[D].Tianjin:Tianjin University of Science and Technology, 2014.
[24] XU Z L, SHEN Y D, BEIER R C, et al.Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants:A review[J].Analytica Chimica Acta, 2009, 647(2):125-136.
[25] AHN K C, WATANABE T, GEE S J, et al.Hapten and antibody production for a sensitive immunoassay determining a human urinary metabolite of the pyrethroid insecticide permethrin[J].Journal of Agricultural & Food Chemistry, 2004, 52(15):4 583-4 594.
[26] HUANG J J, LIU J, LIU J X, et al.A microtitre chemiluminescence sensor for detection of pyrethroids based on dual-dummy-template molecularly imprinted polymer and computational simulation[J].Luminescence, 2020, 35(1):120-128.
[27] 王兰东, 冯东晓, 张淑敏.纳米抗体研究进展[J].生物技术通讯, 2016, 27(3):453-458.
WANG L D, FENG D X, ZHANG S M. Research progress of nanobody[J]. Letters in Biotechnology, 2016, 27(3):453-458.
[28] 刘静, 胡耀中, 黄鹤.纳米抗体用于肿瘤诊断与治疗的研究进展[J].化学工业与工程, 2013, 30(4):29-35;54.
LIU J, HU Y Z, HUANG H. Research progress of nanobodies in diagnosis and therapy of tumor[J]. Chemical Industry and Engineering, 2013, 30(4):29-35; 54.
[29] ERTÜRK G, MATTIASSON B.Molecular imprinting techniques used for the preparation of biosensors[J].Sensors, 2017, 17(2):288.
[30] 季芯羽, 叶泰, 袁敏, 等. 菊酯类农药代谢物分子印迹荧光传感器的制备及应用[J]. 分析测试学报, 2019, 38(12):1 487-1 492.
JI X Y, YE T, YUAN M, et al. Preparation and application of a molecularly imprinted fluorescence sensor for pyrethroid pesticide metabolite[J]. Journal of Instrumental Analysis, 2019, 38(12):1 487-1 492.
[31] CAI Y, HE X, CUI P L, et al.Molecularly imprinted microspheres based multiplexed fluorescence method for simultaneous detection of benzimidazoles and pyrethroids in meat samples[J].Food Chemistry, 2020, 319:126539.
[32] COSTA E, CLIMENT E, AST S, et al.Development of a lateral flow test for rapid pyrethroid detection using antibody-gated indicator-releasing hybrid materials[J].The Analyst, 2020, 145(10):3 490-3 494.
[33] FENG J, SHAN G M, HAMMOCK B D, et al.Fluorescence quenching competitive immunoassay in micro droplets[J].Biosensors and Bioelectronics, 2003, 18(8):1 055-1 063.
[34] 刘景坤. 农产品中氰戊菊酯农药残留免疫检测技术研究[D].北京:中国农业科学院, 2012.
LIU J K.Immunoassay research for fenvalerate residue in agro-product[D].Beijing:Chinese Academy of Agricultural Sciences, 2012.
[35] XIAO T T, SHI X Z, JIAO H F, et al.Selective and sensitive determination of cypermethrin in fish via enzyme-linked immunosorbent assay-like method based on molecularly imprinted artificial antibody-quantum dot optosensing materials[J].Biosensors and Bioelectronics, 2016,75:34-40.
[36] 邱浩. 硅基荧光表面印迹复合材料的制备及其靶向检测环境中拟除虫菊酯的研究[D]. 镇江: 江苏大学, 2017.
QIU H. Preparation of the silicon-based fluorescent surface imprinted composite materials and research on target detection of pyrethroids in the environment[D]. Zhenjiang: Jiangsu University, 2017.
[37] FRUHMANN P, SANCHIS A, MAYERHUBER L, et al.Immunoassay and amperometric biosensor approaches for the detection of deltamethrin in seawater[J].Analytical & Bioanalytical Chemistry, 2018, 410(23):5 923-5 930.
[38] WANG M R, KANG H M, XU D, et al.Label-free impedimetric immunosensor for sensitive detection of fenvalerate in tea[J].Food Chemistry, 2013, 141(1):84-90.
[39] EL-MOGHAZY A Y, HUO J Q, AMALY N, et al.An innovative nanobody-based electrochemical immunosensor using decorated nylon nanofibers for point-of-care monitoring of human exposure to pyrethroid insecticides[J].ACS Applied Materials & Interfaces, 2020, 12(5):6 159-6 168.
[40] 赵颖, 王双节, 柳颖, 等.毒死蜱等10种农药多残留快速检测芯片研究[J].分析化学, 2019, 47(11):1 759-1 767.
ZHAO Y, WANG S J, LIU Y, et al.Immunochip assay for multi-residue rapid detection of ten kinds of common pesticides[J].Chinese Journal of Analytical Chemistry, 2019, 47(11):1 759-1 767.
[41] 兰美静. 农药多残留免疫芯片-纳米金增敏型检测体系构建[D]. 杭州: 浙江大学, 2015.
LAN M J. Establishment of an immunochip for multi-pesticides determination based on colloidal gold enhancement[D]. Hangzhou: Zhejiang University, 2015.
[42] 左晓维, 雷琳, 刘河冰, 等.荧光免疫分析法检测食品中黄曲霉毒素的研究进展[J].食品与发酵工业, 2019,45(1):236-245.
ZUO X W, LEI L, LIU H B, et al. Research progress on detecting aflatoxins in foods using fluorescence immunoassay[J]. Food and Fermentation Industries, 2019, 45(1):236-245.
[43] ZHENG H, ZHAN X Q, BIAN Q N, et al.Advances in modifying fluorescein and rhodamine fluorophores as fluorescent chemosensors[J].Chemical Communications, 2013, 49(5):429-447.
[44] JAHANGIR M A, GILANI S J, MUHEEM A, et al.Quantum dots:Next generation of smart nano-systems[J].Pharmaceutical Nanotechnology, 2019, 7(3):234-245.
[45] 王馨, 胡文忠, 陈晨, 等.纳米材料在食源性致病菌检测中的应用[J].食品与发酵工业, 2016, 42(6):243-247.
WANG X, HU W Z, CHEN C, et al.Application of nanomaterials in detection of foodborne pathogenic bacteria[J].Food and Fermentation Industries, 2016, 42(6):243-247.
[46] 朱小钿, 张燕, 彭宏威, 等.免疫传感器在食品安全检测中的应用[J].食品安全质量检测学报, 2019, 10(3):626-632.
ZHU X T, ZHANG Y, PENG H W, et al.Application of immunosensors in food safety detection[J].Journal of Food Safety and Quality, 2019, 10(3):626-632.
[47] 曲信芹, 凌红丽, 蒋贻海, 等.免疫芯片技术在兽药残留检测中的应用[J].中国动物检疫, 2015, 32 (10):21-24.
QU X Q, LING H L, JIANG Y H, et al. Application of immunochips in veterinary drug residue detection[J]. China Animal Health Inspection, 2015, 32(10):21-24.
[48] 张煜超, 王芳芳, 李周敏, 等.小分子药物人工抗原的合成与鉴定研究进展[J].药物分析杂志, 2014, 34(6):947-951.
ZHANG Y C, WANG F F, LI Z M, et al. Research progress of artificial antigen synthesis and identification for micromolecule drug[J]. Chinese Journal of Pharmaceutical Analysis, 2014, 34(6):947-951.
[49] SHRIVER-LAKE L C, GOLDMAN E R, DEAN S N, et al.Lipid-tagged single domain antibodies for improved enzyme-linked immunosorbent assays[J].Journal of Immunological Methods, 2020, 481-482:112790.
[50] NISTLER A, NIESSNER R, SEIDEL M.Magnetic nanocomposites:Versatile tool for the combination of immunomagnetic separation with flow-based chemiluminescence immunochip for rapid biosensing of Staphylococcal enterotoxin B in milk[J].Analytical and Bioanalytical Chemistry, 2019, 411(19):4 951-4 961.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.