Recently, various biosensors based on nucleic acid aptamers have been applied to the detection of mycotoxins with high sensitivity, but the dynamic flexibility of aptamer structure can easily lead to false positive and low recognition ability, which limits the application of aptamer sensing technology. To solve this problem, a complete aptamer was split into two aptamer fragments to reduce the structural flexibility of aptamers and stabilize its structure, and a dual-signal electrochemical sensor based on split aptamers was constructed for the detection of AFM1. In this work, AFM1 aptamer was split into two segments (S1 and S2). S1 was modified with the thiol group, and then was immobilized on the electrode surface by Au-S. S2 was modified with methylene blue (MB) as the sensing signal, and the complementary chain CS1 of S1 was modified with ferrocene (Fc) as another sensing signal. Without AFM1, a double chain structure was formed by S1 and CS1, and the Fc signal enhanced. In the presence of AFM1, CS1 was released, and S1 and S2 formed a certain conformation to recognize AFM1. The intensity of Fc decreased, and the intensity of MB increased. The results showed that the linear range of this sensor was 0.050-0.800 μg/L with the minimum detection limit of 0.015 μg/L, and the sensor had a good selectivity. This method provides a new idea to solve the problem of aptamer stability.
DU Congcong
,
GUO Ting
,
ZHOU Hongyuan
,
LIU Xiaozhu
,
ZHANG Yuhao
,
MA Liang
. An electrochemical sensor based on split aptamer and dual signal for the detection of aflatoxin M1[J]. Food and Fermentation Industries, 2022
, 48(10)
: 22
-27
.
DOI: 10.13995/j.cnki.11-1802/ts.030026
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