Food and Fermentation Industries >

2022 , Vol. 48 >Issue 10: 101 - 105

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028844

Bacteriostasis of Coptis on intestinal pathogens in vitro

  • JIANG Lishi ,
  • ZUO Xifeng ,
  • ZHANG Xinming ,
  • LUO Yuan ,
  • LI Xinai ,
  • GUO Wenxiu ,
  • DENG Yun
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  • 1(College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611100, China)
    2(College of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu 611100, China)
    3(College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611100, China)

Received date: 2021-08-10

  Revised date: 2021-09-28

  Online published: 2022-06-10

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Abstract

Coptis herbs has significant antibacterial activity and has great potential in the development of natural food preservatives. However, there has been no report on the anti-bacteriostasis of different varieties and processed Coptis on common intestinal pathogens in food, and the pharmacodynamic material basis is not completely clear. The methanol extracts(ME)of raw and wine-processed Coptis chinensis Franch. and Coptis deltoidea C.Y. Cheng et Hisao were obtained by ultrasonic-assisted extraction with 70% methanol, and then the petroleum ether extract (PE), ethyl acetate extract (EAE), n-butanol extract (BE), water extract (WE) of the four herbs were extracted in turn. The antibacterial activity of extracts from different polar parts was determined by filter paper method, and the minimum inhibitory concentration (MIC) was determined by double dilution method. The experimental results showed that C. chinensis had a better inhibitory effect on Staphylococcus aureus and Yersinia enterocolitica, while C. deltoidea had a stronger inhibitory effect on Enterobacter cloacae. The antibacterial activity of both wine-processed Coptis herbs against Klebsiella pneumoniae was significantly enhanced. The strongest antibacterial activity of Coptis herbs was methanol extract, followed by n-butanol extract and water extract. The MIC value of Coptis herbs ranged from 3.125 to 25 mg/mL. The variety and processing had certain influence on the antibacterial activity of Coptis.

Key words： Coptis chinensis; Coptis deltoidea; processed products; polarity part; enteropathogenic bacteria

Cite this article

JIANG Lishi , ZUO Xifeng , ZHANG Xinming , LUO Yuan , LI Xinai , GUO Wenxiu , DENG Yun . Bacteriostasis of Coptis on intestinal pathogens in vitro[J]. Food and Fermentation Industries, 2022 , 48(10) : 101 -105 . DOI: 10.13995/j.cnki.11-1802/ts.028844

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