Composite modified millet starch prepared by hydrophobic modification of octenyl succinic acid (OSA) and ball milling was used to stabilize Pickering emulsion and loaded with β-carotene. The stability of Pickering emulsion was confirmed at different pH salt and storage conditions. The loading and release characteristics of active substances in a complex environment were evaluated by dialysis experiment, storage experiment and simulated human gastrointestinal digestion experiment. The results showed that composite modified millet starch Pickering emulsion loaded with β-carotene showed good pH, salt ion and storage stability. The initial loading rate of β-carotene in Pickering emulsion of composite modified millet starch was 92.92%, which was significantly higher than that of OSA starch with single modified (82.44%). After 15 days, the loading rate of β-carotene in the Pickering emulsion of composite modified millet starch was as follows: 71.71% (4 ℃, light) >62.60% (25 ℃, light) >58.25% (25 ℃, light) >51.97% (37 ℃, light), which was due to adverse conditions such as light and high temperature. Dialysis release and simulated gastrointestinal digestion in vitro experimental results showed that Pickering emulsion stabilized by compound modified millet starch had a strong protective effect on β-carotene, effectively reduced the releasing rate, and increased the retention rate of β-carotene.
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