Food and Fermentation Industries >

2022 , Vol. 48 >Issue 10: 226 - 233

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028425

Determination of glucose, fructose and sucrose in fruit juice by 1H-NMR

  • FENG Cuiping ,
  • LIU Yinuo ,
  • XU Ziwei ,
  • FAN Shuangxi ,
  • YUE Hongwei ,
  • XIN Gang ,
  • JIANG Nan ,
  • ZHONG Qiding
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  • 1(Sinolight Food Inspection and Certification Co.Ltd., Beijing 100015, China)
    2(College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100091, China)
    3(SDIC Zhonglu Fruit Juice Co.Ltd., Beijing 100070, China)
    4(Rushan R & D Center of SDIC Zhonglu Fruit Juice Co.Ltd., Weihai 264513, China)
    5(National Food Quality Inspection and Testing Center, Beijing 100015, China)

Received date: 2021-06-23

  Revised date: 2021-09-14

  Online published: 2022-06-10

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Abstract

In this study, potassium hydrogen phthalate and citric acid were used respectively as the internal and external standards for the quantitative determination of glucose, fructose and sucrose in juice by water signal suppressing 1H NMR. The results showed that the limit of detection and quantification of fructose were 0.005 g/L and 0.018 g/L, respectively. The limit of detection and quantification of glucose were 0.006 g/L and 0.021 g/L, respectively. Moreover, the limit of detection and quantification of sucrose were 0.009 g/L and 0.030 g/L, respectively. Good linearity was observed in the range of 0.025-6.40 g/L, with correlation coefficient R2 greater than 0.999. The spiked recoveries of the external standard method were 100.9%-104.7% and the spiked recoveries of internal standard method were 102.1%-108.6%. Besides, the precisions (relative standard deviation, RSD) were less than 1%; The method had good reproducibility and accurate results. The error between NMR and HPLC of GB 5009.8—2016 was within ±5%, which met the requirements of feasibility comparative analysis and validation. Compared with the HPLC method, the method developed here was simple and accuracy. It could save time and labor greatly, and could be used to measure glucose, fructose and sucrose content simultaneously without separation. It can also be widely used in juice quality control and laid a good foundation for the establishment of NMR fingerprint authenticity identification technology of juice.

Key words： juice; glucose; fructose; sucrose; 1H NMR; quantitation

Cite this article

FENG Cuiping , LIU Yinuo , XU Ziwei , FAN Shuangxi , YUE Hongwei , XIN Gang , JIANG Nan , ZHONG Qiding . Determination of glucose, fructose and sucrose in fruit juice by 1H-NMR[J]. Food and Fermentation Industries, 2022 , 48(10) : 226 -233 . DOI: 10.13995/j.cnki.11-1802/ts.028425

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