Riboflavin is one of the essential nutrients for human, and guanosine triphosphate (GTP) is an important precursor of riboflavin biosynthesis. In order to further increase riboflavin production on the basis of industrial producers, a riboflavin-producing strain Bacillus subtilis S1 was investigated. The purine synthesis pathway transcription repressor gene and GMP reductase gene were inactivated by CRISPR/Cas9-mediated genomic base editing technology, and a gene ribA from Shewanella oneidensis was overexpressed with pvegI promoter. The results showed that the production of riboflavin increased to 3.04 g/L with the inactivation of guaC gene in B. subtilis S1, which blocked the complementary pathway from GMP to IMP and accumulated GMP. The inactivation of purR gene had no effect. The production of riboflavin increased to 3.33g/L with the overexpressed ribA in B. subtilis S1. The production of riboflavin reached 3.43g/L with the inactivated guaC gene and the overexpressed ribA in B. subtilis S1, which increased 22% compared with B. subtilis S1.
GU Zhenyu
,
XIA Miaomiao
,
SU Yuan
,
LIU Chuan
,
LUO Huajun
,
ZHANG Dawei
. Metabolic engineering modifies purine synthesis pathway to increase riboflavin production[J]. Food and Fermentation Industries, 2022
, 48(11)
: 10
-15
.
DOI: 10.13995/j.cnki.11-1802/ts.029360
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