Screening and breeding the strain with high lipase activity, and the content changes of phospholipids, i.e.phosphatidylcholine (PC), phosphatidylinositol (PI) and phosphatidylethanolamine (PE) in Douchi were investigated. The Victoria blue and neutral red lipase plates were used to preliminary screen the strains with high lipase activity from Semen Sojae Praeparatum, the P-nitrophenol method was used to re-screen the strains, and atmospheric and room temperature plasma (ARTP) was adopted to mutagenize the selected strain to improve its enzyme activity. The Douchi was prepared using black soybean with different starters (original strain and mutant), the contents of PC, PI and PE in Douchi at different fermentation stages were detected by HPLC. A strain with high lipase activity was screened out, and the enzyme activity of the strain after treated by ARTP increased by 25.98%, and it was identified as Absidia corymbifera by Biolog microbial identification system. There is no significant difference of the contents of PC, PI and PE in Douchi fermented 7 d by the original and mutated strains, however, the contents of PC, PI and PE in Douchi fermented by the selected strains were 5 times, 8-11 times and 30 times than that of Semen Sojae Praeparatum. The strain with high lipase activity was selected, the content of phospholipid in Douchi improved significantly, and the preparation process of Douchi with rich phospholipid was determined, which lays a foundation for subsequent research.
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