Metschnikowia pulcherrima is a common non-Saccharomyces cerevisiae with a variety of enzyme activities. It has excellent brewing characteristics in reducing alcohol content, increasing glycerol content and improving wine flavor and quality. Therefore, M. pulcherrima has great potential in wine brewing. Strain Mp 01 is a M. pulcherrima with excellent properties in reducing alcohol and high glycerol content, which was screened in preliminary study. To prepare high active dried cell mass, the optimal harvest time and vacuum freeze-dried composite protective agent formula for strain Mp 01 were optimized. The results indicated that the optimal harvest time was 24 h, then the yeast was centrifuged at 2 852×g for 15 min, and 93.55% of the yeast was collected. Moreover, the optimized formula of the vacuum freeze-drying protective agent was 130 g/L of maltose, 100 g/L of sucrose and 140 g/L of trehalose. Under the optimal conditions, the maximum survival rate of strain Mp 01 was 94.92%, which was 16% and 10% higher than that of the best single protective agent and combined protective agent before optimization, respectively. This study provides a compound protective agent for the preparation of M. pulcherrima, and provides a research basis and reference for the development of other yeast freeze-dried protective agents.
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