Based on natural deep eutectic solvent (DES), microwave-assisted green extraction was applied for production of polysaccharides from Spirulina platensis. After partial separation and purification of the polysaccharides extracted by DES, antioxidant and anticancer activity in vitro were evaluated. Nine different combinations of deep eutectic solvents were evaluated for extraction of polysaccharides from S. platensis, and the results showed that DES system composed of choline chloride and 1,4-butanediol with molar ratio of 1:4 possessed the optimal extraction efficiency for polysaccharides. The maximum yields of polysaccharides109.80 mg/g was attained at 81 ℃, extracted for 28 min and solid-liquid ratio of 20 mg/mL, with DES water content of 29%. The results revealed that polysaccharides extracted from S. platensis exhibited excellent antioxidant activities in vitro, including DPPH and ABTS radical-scavenging activity. For human cervical cancer HeLa cells, the polysaccharides possessed a strong inhibition effect on the growth rate. Thus, DES, as an environmentally-friendly solvent, can be applied to extract polysaccharides from plant materials.
LUO Guanghong
,
WANG Hairong
,
CUI Jing
,
ZHANG Xifeng
,
HE Lijie
,
WEI Rui
. Microwave-assisted deep eutectic solvents extraction, partial purification and in vitro biological activity of polysaccharides from Spirulina platensis[J]. Food and Fermentation Industries, 2022
, 48(11)
: 107
-113
.
DOI: 10.13995/j.cnki.11-1802/ts.029283
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