Food and Fermentation Industries >

2022 , Vol. 48 >Issue 12: 188 - 195

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028102

The development of SSR markers and construction of fingerprint library for edible daylily

  • LIU Xinxing ,
  • OU Qiaoming ,
  • LUO Junjie ,
  • LI Zhongwang ,
  • SHI Youtai ,
  • CUI Wenjuan
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  • (Biotechnology Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China)

Received date: 2021-05-26

  Revised date: 2021-08-17

  Online published: 2022-07-15

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Abstract

The SSR markers were developed by transcriptome sequencing technology to provide technical support for the identification, evaluation and genetic diversity analysis of edible daylily germplasm resources. The results showed that a total of 104 177 unigenes containing 79 106 833 bpin sequence, with an average length of 759 bp was obtained. The Unigene was searched using MISA software, and a total of 15 890 SSRs were detected which including single nucleotides, dinucleotides and trinucleotides accounting for a relatively high proportion of 18.57%, 27.23% and 44.26%, respectively. A total of 114 pairs of SSR primers were used for the screening, and finally, 20 pairs of primers were screened out by polyacrylamide gel electrophoresis. A total of 88 allelic variants were detected in 43 daylily germplasms, and the number of allelic variants at each locus was 3-8. The polymorphism information content (PIC) was ranged from 0.23 to 0.77. Based on the fragment size of the amplified product, the allelic variation of each SSR locus and the corresponding reference variety were determined, and a DNA fingerprint identification technology system was constructed. The SSR markers developed in this study are rich in polymorphism.

Key words： daylily; SSR marker; fingerprint

Cite this article

LIU Xinxing , OU Qiaoming , LUO Junjie , LI Zhongwang , SHI Youtai , CUI Wenjuan . The development of SSR markers and construction of fingerprint library for edible daylily[J]. Food and Fermentation Industries, 2022 , 48(12) : 188 -195 . DOI: 10.13995/j.cnki.11-1802/ts.028102

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