In this study, analytical method of four tocopherols in oilseeds and edible oils was developed by reversed-phase high performance liquid chromatography. The sample was firstly dissolved by isopropanol and 0.1 g antioxidant (BHT) was added, then the mixture was extracted by vortex, ultrasonication and centrifugation. Finally, the mixture passed through 0.22 μm organic filter membrane for the purification. Poroshell 120 SB-C18 liquid chromatographic column was used with 90% methanol-water as mobile phase for the detection of tocopherols. The validation results indicated that the complete separation of four tocopherols could be achieved within 10 min by quantitative analysis on a high performance liquid chromatography with a flow rate of 0.75 mL/min, a column temperature of 30 ℃ and a detection wavelength of 294 nm. The recoveries of the four tocopherols were in the range of 87%-109%, and the precision (RSD) was in the range of 1.88%-9.89%. The limits of detection (LODs) for α-tocopherol, β-tocopherol, γ-tocopherol and δ-tocopherol were 0.16, 0.21, 0.20 and 0.22 μg/g, and the limits of quantification (LOQs) were 0.53, 0.68, 0.66 and 0.72 μg/g respectively. The correlation coefficients were higher than 0.999 2. This method has a simple extraction process, high extraction efficiency and sensitivity, which meets the actual sample detection requirements.
ZHANG Yu
,
WANG Xueyan
,
WANG Xuefang
,
QI Xin
,
MA Fei
,
YU Li
,
ZHANG Liangxiao
,
LI Peiwu
. Determination of tocopherols in oilseeds by reversed phase high performance liquid chromatography[J]. Food and Fermentation Industries, 2022
, 48(12)
: 243
-248
.
DOI: 10.13995/j.cnki.11-1802/ts.030954
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