Food and Fermentation Industries >

2022 , Vol. 48 >Issue 12: 261 - 266

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028088

Lateral flow colloidal gold immunoassay paper for rapid determination of tylosin in milk

  • XING Guangxu ,
  • SUN Xuefeng ,
  • ZHAO Dong ,
  • YANG Jifei ,
  • ZHAO Yali ,
  • ZHANG Gaiping
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  • 1(Henan Key Laboratory for Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
    2(College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China)

Received date: 2021-05-26

  Revised date: 2021-07-16

  Online published: 2022-07-15

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Abstract

Macrolide antibiotics are a very important class of antibacterial compounds, and tylosin (TYL) is the most commonly used premixed macrolide antibiotics. However, the residues of these drugs can cause allergic reactions in the human body and become resistant to antibiotics. In order to evaluate the residues of TYL, sensitive analytical methods are required to ensure the determination at low concentrations. In this work, we developed a rapid immunochromatographic lateral flow test strip for the determination of TYL residues in milk samples. We screened anti-TYL monoclonal antibodies. The antibody titer was 2.56×10-5, and the IC50 was 4.609 1 ng/mL. The test strip was composed of a sample pad, a conjugate pad, a control line and a test line, and a NC membrane and an absorbent pad. Different standard samples (0, 2, 4, 8, 16, 32, and 64 ng/mL) of TYL were measured with test strips. The limit of detection (LOD) of the test paper calculated with the scanner was 1.543 4 ng/mL, while LOD calculated by naked eye was 10 ng/mL, and the IC50 was calculated to be 7.836 2 ng/mL. No special equipment was required. The test result could be obtained within 20 minutes. The parallel analysis of milk showed that the results obtained from the test strips and HPLC were comparable, and the test strips did not cross-react with other veterinary drugs. Therefore, this test strip is very simple and fast, and can be used as a screening method for quantitative, semi-quantitative or qualitative detection of TYL residues in milk.

Key words： milk; tylosin; monoclonal antibody; immunochromatographic; lateral flow strip

Cite this article

XING Guangxu , SUN Xuefeng , ZHAO Dong , YANG Jifei , ZHAO Yali , ZHANG Gaiping . Lateral flow colloidal gold immunoassay paper for rapid determination of tylosin in milk[J]. Food and Fermentation Industries, 2022 , 48(12) : 261 -266 . DOI: 10.13995/j.cnki.11-1802/ts.028088

References

[1] NASR J J, SHALAN S, BELAL F.Simultaneous determination of tylosin and josamycin residues in muscles, liver, eggs and milk by MLC with a monolithic column and time-programmed UV detection:Application to baby food and formulae[J].Chemistry Central Journal, 2014, 8(1):1-9.
[2] MECHESSO A F, PARK S C.Tylosin exposure reduces the susceptibility of Salmonella Typhimurium to florfenicol and tetracycline[J].BMC Veterinary Research, 2020, 16(1):22.
[3] KIM J, GUEVARRA R B, NGUYEN S G, et al.Effects of the antibiotics growth promoter tylosin on swine gut microbiota[J].Journal of Microbiology and Biotechnology, 2016, 26(5):876-882.
[4] ABDALLAH A, ZHANG P, ZHONG Q Z, et al.Application of traditional Chinese herbal medicine by-products as dietary feed supplements and antibiotic replacements in animal production[J].Current Drug Metabolism, 2019, 20(1):54-64.
[5] PENG D P, FENG L, PAN Y H, et al.Development and validation of an indirect competitive enzyme-linked immunosorbent assay for monitoring organoarsenic compounds in edible chicken and pork and feed[J].Food Chemistry, 2016, 197:821-828.
[6] DE FREITAS A G, DE MAGALHÃES B E, MINHO L A, et al.FTIR spectroscopy with chemometrics for determination of tylosin residues in milk[J].Journal of the Science of Food and Agriculture, 2021, 101(5):1 854-1 860.
[7] SEHATI N, DALALI N, SOLTANPOUR S, et al.Extraction and preconcentration of tylosin from milk samples through functionalized TiO2 nanoparticles reinforced with a hollow fiber membrane as a novel solid/liquid-phase microextraction technique[J].Journal of Separation Science, 2014, 37(15):2 025-2 031.
[8] CIVITAREALE C, FIORI M, BALLERINI A, et al.Identification and quantification method of spiramycin and tylosin in feedingstuffs with HPLC-UV/DAD at 1 ppm level[J].Journal of Pharmaceutical and Biomedical Analysis, 2004, 36(2):317-325.
[9] OZDEMIR Z, TRAS B, UNEY K.Distribution of hydrophilic and lipophilic antibacterial drugs in skim milk, cream, and casein[J].Journal of Dairy Science, 2018, 101(12):10 694-10 702.
[10] VINCENT U, GIZZI G, HOLST C, et al.Validation of an analytical method for the determination of spiramycin, virginiamycin and tylosin in feeding-stuffs by thin-layer chromatography and bio-autography[J].Food Additives and Contaminants, 2007, 24(4):351-359.
[11] PATYRA E, NEBOT C, GAVILÁN R E, et al.Development and validation of an LC-MS/MS method for the quantification of tiamulin, trimethoprim, tylosin, sulfadiazine and sulfamethazine in medicated feed[J].Food Additives & Contaminants:Part A, 2018, 35(5):882-891.
[12] JANK L, MARTINS M T, ARSAND J B, et al.High-throughput method for macrolides and lincosamides antibiotics residues analysis in milk and muscle using a simple liquid-liquid extraction technique and liquid chromatography-electrospray-tandem mass spectrometry analysis (LC-MS/MS)[J].Talanta, 2015, 144:686-695.
[13] HUANG J X, YAO C Y, YANG J Y, et al.Design of novel haptens and development of monoclonal antibody-based immunoassays for the simultaneous detection of tylosin and tilmicosin in milk and water samples[J].Biomolecules, 2019, 9(12):770.
[14] HENDRICKSON O D, ZVEREVA E A, ZHERDEV A V, et al.Development of a double immunochromatographic test system for simultaneous determination of lincomycin and tylosin antibiotics in foodstuffs[J].Food Chemistry, 2020, 318:126510.
[15] LE T, ZHU L Q, YANG X.A quantum dot-based immunoassay for screening of tylosin and tilmicosin in edible animal tissues[J].Food Additives & Contaminants:Part A, 2015, 32(5):719-724.
[16] CHEN W Y, HUANG Z, HU S, et al.Invited review:Advancements in lateral flow immunoassays for screening hazardous substances in milk and milk powder[J].Journal of Dairy Science, 2019, 102(3):1 887-1 900.
[17] SHI Q Q, HUANG J, SUN Y N, et al.Utilization of a lateral flow colloidal gold immunoassay strip based on surface-enhanced Raman spectroscopy for ultrasensitive detection of antibiotics in milk[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2018, 197:107-113.
[18] RYU J H, KWON M, MOON J D, et al.Development of a rapid automated fluorescent lateral flow immunoassay to detect hepatitis B surface antigen (HBsAg), antibody to HBsAg, and antibody to hepatitis C[J].Annals of Laboratory Medicine, 2018, 38(6):578-584.
[19] MARTISKAINEN I, JUNTUNEN E, SALMINEN T, et al.Double-antigen lateral flow immunoassay for the detection of anti-HIV-1 and-2 antibodies using upconverting nanoparticle reporters[J].Sensors, 2021, 21(2):330.
[20] LI G, WANG A P, CHEN Y M, et al.Development of a colloidal gold-based immunochromatographic strip for rapid detection of severe acute respiratory syndrome coronavirus 2 spike protein[J].Frontiers in Immunology, 2021, 12:635677.
[21] LI C Z, VANDENBERG K, PRABHULKAR S, et al.Paper based point-of-care testing disc for multiplex whole cell bacteria analysis[J].Biosensors and Bioelectronics, 2011, 26(11):4 342-4 348.
[22] ZHANG G P, GUO J Q, WANG X N, et al.Development and evaluation of an immunochromatographic strip for trichinellosis detection[J].Veterinary Parasitology, 2006, 137(3-4):286-293.
[23] VERHEIJEN R, STOUTEN P, CAZEMIER G, et al.Development of a one step strip test for the detection of sulfadimidine residues[J].The Analyst, 1998, 123(12):2 437-2 441.
[24] LI Z X, ZHAO F P, TANG T T, et al.Development of a colloidal gold immunochromatographic strip assay for rapid detection of bovine rotavirus[J].Viral Immunology, 2019, 32(9):393-401.
[25] NA G Q, HU X F, YANG J F, et al.A rapid colloidal gold-based immunochromatographic strip assay for monitoring nitroxynil in milk[J].Journal of the Science of Food and Agriculture, 2020, 100(5):1 860-1 866.
[26] ZHANG Y Y, HE F Y, WAN Y P, et al.Generation of anti-trenbolone monoclonal antibody and establishment of an indirect competitive enzyme-linked immunosorbent assay for detection of trenbolone in animal tissues, feed and urine[J].Talanta,2011, 83(3):732-737.
[27] ZHANG G P, WANG X N, YANG J F, et al.Development of an immunochromatographic lateral flow test strip for detection of β-adrenergic agonist clenbuterol residues[J].Journal of Immunological Methods, 2006, 312(1-2):27-33.
[28] WANG Z X, GUO L L, LIU L Q, et al.Colloidal gold-based immunochromatographic strip assay for the rapid detection of three natural estrogens in milk[J].Food Chemistry, 2018, 259:122-129.
[29] 徐凤琴, 朱群艳, 张婳, 等.血清中可溶性白介素-2受体胶体金免疫层析试纸检测方法研究[J].分析化学, 2021, 49(6):973-981.
XU F Q, ZHU Q Y, ZHANG H, et al.Preparation of collidal gold-labeled immunochromatographic strips for detection method of soluble interleukin-2 receptors in real samples[J].Chinese Journal of Analytical Chemistry, 2021, 49(6):973-981.
[30] TANG Q Y, ZHANG C X.Data Processing System (DPS) software with experimental design, statistical analysis and data mining developed for use in entomological research[J].Insect Science, 2013, 20(2):254-260.
[31] HU D F, FULTON B, HENDERSON K, et al.Identification of tylosin photoreaction products and comparison of ELISA and HPLC methods for their detection in water[J].Environmental Science & Technology, 2008, 42(8):2 982-2 987.
[32] GAUDIN V, HEDOU C, VERDON E.Validation of two ELISA kits for the screening of tylosin and streptomycin in honey according to the European decision 2002/657/EC[J].Food Additives & Contaminants:Part A, 2013, 30(1):93-109.
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