In this study, 114 strains of Lactiplantibacillus plantarum isolated from Sichuan pickles were screened by Oxford cup double-layer plate method, which had strong antagonistic activity against Escherichia coli O157∶H7, and the characteristics of protein antibacterial substances produced by L. plantarum were studied. The results showed that the supernatant of 114 strains of L. plantarum could inhibit E. coli, and 16 strains of L. plantarum could still inhibit E. coli after acid drainage, and the inhibitory effect was not affected by catalase, but was significantly reduced or even disappeared after protease treatment. Further screening showed that the antibacterial substances produced by L. plantarum IMAU80122 had the characteristics of acid resistance (pH 3) and weak alkali resistance (pH 8), had certain stability at 80 ℃, and had good resistance to different surfactants, organic solvents, metal ions, salt concentration and UV irradiation time. The antibacterial activity against G- strain was stronger than G+ strain, but it had no antibacterial activity against tested lactic acid bacteria. In conclusion, the bacteriostatic substances produced by L. plantarum IMAU80122 have protein properties, which not only have strong bacteriostatic activity, but also have good stability and broad-spectrum bacteriostatic effect, and have certain development and application potential in food processing and pharmaceutical industry.
ZHAI Jialin
,
ZHAO Jingna
,
WANG Dandan
,
ZHA Musu
,
CHEN Yongfu
. Screening of Lactiplantibacillus plantarum with bacteriostatic activity and study on properties of bacteriostatic substances[J]. Food and Fermentation Industries, 2022
, 48(13)
: 84
-90
.
DOI: 10.13995/j.cnki.11-1802/ts.029710
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