Food and Fermentation Industries >

2022 , Vol. 48 >Issue 13: 91 - 102

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029607

Polygonatum kingianum Coll. et Hemsl water extracts combined with intermittent fasting alleviates high fat diet-induced obesity and liver injury by modulating gut microbiome

  • WEI Yucheng ,
  • YANG Minmin ,
  • SHI Lin ,
  • LIU Tianqi ,
  • YAN Tao ,
  • ZHANG Yan ,
  • LIANG Yifan ,
  • ZHOU Lanqi ,
  • ZOU Jiale ,
  • ZHANG Huafeng
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  • 1(School of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, China)
    2(National Engineering Laboratory for Resources Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi’an 710119, China)
    3(International Joint Research Center of Shaanxi Province for Food and Health Sciences, Shaanxi Normal University, Xi’an 710119, China)
    4(Key Laboratory of Characteristic Fruit Storage and Preservation, Shaanxi Normal University, Xi’an 710119, China)

Received date: 2021-10-05

  Revised date: 2021-11-01

  Online published: 2022-08-03

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Abstract

The aim of this study was to investigate the effect of water extract from Polygonatum kingianum Coll. et Hemsl (PK) combined with intermittent fasting (IF) on preventing abnormal weight gain and hepatic steatosis in mice with high fat diet by modulating gut microbiota. PK contained 69.71% total polysaccharide and 5.4% total polyphenols. A total of 325 metabolites was detected by using liquid chromatograph-mass spectrometer based metabolomics, mainly including prebiotic oligosaccharides (e.g. raffinose, stachyose and isomaltose), amino acids (e.g. γ-aminobutyric acid, arginine, tryptophan and serine), and other compounds showing health-promoting effects, (e.g. betaine). The 8-week-old (n=60) healthy male C57BL/6J mice were randomly assigned into 6 groups: normal chow (NC), NC with oral administration of PK (NC+PK), NC with oral administration of PK and intermittent fasting (NC+PK+IF), high fat diet (HF), HF+PK and HF+PK+IF, respectively. During the intervention of 8 weeks, PK and PK+IF effectively prevented high-fat-induced abnormal weight gain, improved glucose tolerance and superoxide dismutase activity in liver, reduced serum triglycerides, and alleviated HF-induced liver cell degeneration and abnormal lipid accumulation. Moreover, PK significantly increased relative abundance of Bacteroidetes and decreased the ratio of Firmicutes to Bacteroidetes. Both PK and PK+IF intervention inhibited HF-induced upregulation in Clostridium, Escherichia-Shigella, Acinetobacter, and Ruminococcus at genus level, of which have been associated with obesity, excessive lipid accumulation and liver damage; conversely, PK and PK+IF elevated short-chain fatty acid-promoting genera, i.e. Alloprevotella, Prevotella, Butyrivibrio, and Bifidobacterium. PK-induced improvements in body weight, glucose tolerance, lipid metabolism and oxidative stress were strongly correlated with PK-altered gut microbiota. In conclusion, P. kingianum water extract rich in various probiotic substances and could beneficially prevent HF-induced obesity and liver damage by modulating gut microbiota.

Key words： Polygonatum kingianum Coll. et Hemsl; intermittent fasting; high fat diet; gut microbiota; liver injury

Cite this article

WEI Yucheng , YANG Minmin , SHI Lin , LIU Tianqi , YAN Tao , ZHANG Yan , LIANG Yifan , ZHOU Lanqi , ZOU Jiale , ZHANG Huafeng . Polygonatum kingianum Coll. et Hemsl water extracts combined with intermittent fasting alleviates high fat diet-induced obesity and liver injury by modulating gut microbiome[J]. Food and Fermentation Industries, 2022 , 48(13) : 91 -102 . DOI: 10.13995/j.cnki.11-1802/ts.029607

References

[1] KATTA N, LOETHEN T, LAVIE C J, et al. Obesity and coronary heart disease:Epidemiology, pathology, and coronary artery imaging[J].Current Problems in Cardiology, 2021, 46(3):100655.
[2] YANG M M, YAN T, YU M, et al.Advances in understanding of health-promoting benefits of medicine and food homology using analysis of gut microbiota and metabolomics[J].Food Frontiers, 2020, 1(4):398-419.
[3] 王硕. 黄精的研究现状[J].农业科技通讯, 2020(1):46-47.
WANG S.Current status of research on Polygonatum sibiricum [J].Bulletin of Agricultural Science and Technology, 2020(1):46-47.
[4] 任洪民, 邓亚羚, 张金莲, 等.药用黄精炮制的历史沿革、化学成分及药理作用研究进展[J].中国中药杂志, 2020, 45(17):4 163-4 182.
REN H M, DENG Y L, ZHANG J L, et al.Research progress on processing history evolution, chemical components and pharmacological effects of Polygonati rhizoma[J].China Journal of Chinese Materia Medica, 2020, 45(17):4 163-4 182.
[5] ERDEM Y, ÖZKAN G, ULUSOY ş, et al.The effect of intermittent fasting on blood pressure variability in patients with newly diagnosed hypertension or prehypertension[J].Journal of the American Society of Hypertension, 2018, 12(1):42-49.
[6] GRAJOWER M M, HORNE B D.Clinical management of intermittent fasting in patients with diabetes mellitus[J].Nutrients, 2019, 11(4):873.
[7] MALINOWSKI B, ZALEWSKA K, WSIERSKA A, et al.Intermittent fasting in cardiovascular disorders-an overview[J].Nutrients, 2019, 11(3):673.
[8] LIN J, JIANG X X, DONG M, et al.Hepatokine pregnancy zone protein governs the diet-induced thermogenesis through activating brown adipose tissue[J].Advanced Science, 2021, 8(21):2101991.
[9] YIN C, LI Z H, XIANG Y L, et al.Effect of intermittent fasting on non-alcoholic fatty liver disease:Systematic review and meta-analysis[J].Frontiers in Nutrition, 2021, 8:709683.
[10] SHI H N,ZHANG B J,ABO-HAMZY T, et al.Restructuring the gut microbiota byintermittent fasting lowers blood pressure[J].Circulation research:a journal of the American Heart Association, 2021, 128(9):1 240-1 254.
[11] CIGNARELLA F, CANTONI C, GHEZZI L, et al.Intermittent fasting confers protection in CNS autoimmunity by altering the gut microbiota[J].Cell Metabolism, 2018, 27(6):1 222-1 235.
[12] VALDES A M, WALTER J, SEGAL E, et al.Role of the gut microbiota in nutrition and health[J].BMJ (Clinical Research Ed.), 2018, 361:k2179.
[13] CANI P D.Microbiota and metabolites in metabolic diseases[J].Nature Reviews Endocrinology, 2019, 15(2):69-70.
[14] KO J H, KWON H S, YOON J M, et al.Effects of Polygonatum sibiricum rhizome ethanol extract in high-fat diet-fed mice[J].Pharmaceutical Biology, 2015, 53(4):563-570.
[15] 朱艳慧, 陈俭双, 文清, 等.高效液相色谱法同时测定金花清感颗粒中14种成分含量[J].中国医院药学杂志, 2021, 41(23):2 451-2 455.
ZHU Y H, CHEN J S, WEN Q, et al.Simultaneous determination of 14 components in Jinhua Qinggan Granules by high performance liquid chromatography[J].Chinese Journal of Hospital Pharmacy, 2021, 41(23):2 451-2 455.
[16] 杨兴鑫, 王曦, 董金材, 等.滇黄精对非酒精性脂肪肝大鼠的保护作用及机制研究[J].中国药学杂志, 2018, 53(12):975-981.
YANG X X, WANG X, DONG J C, et al.Protective effects and mechanisms of Polygonatum kingianum on nonalcoholic fatty liver in rats[J].Chinese Pharmaceutical Journal, 2018, 53(12):975-981.
[17] 马依依, 屈磊, 张帆, 等.脂蛋白肾病肾脏组织油红O染色方法改良[J].临床与实验病理学杂志, 2020, 36(9):1 114-1 115.
MA Y Y, QU L, ZHANG F, et al.Improved oil red O staining method for kidney tissues with lipoprotein nephropathy [J].Chinese Journal of Clinical and Experimental Pathology, 2020, 36(9):1 114-1 115.
[18] RUIZ-OJEDA F J, PLAZA-DÍAZ J, SÁEZ-LARA M J, et al.Effects of sweeteners on the gut microbiota:A review of experimental studies and clinical trials[J].Advances in Nutrition, 2019, 10(suppl_1):S31-S48.
[19] LIU G M, BEI J, LIANG L, et al.Stachyose improves inflammation through modulating gut microbiota of high-fat diet/streptozotocin-induced type 2 diabetes in rats[J].Molecular Nutrition & Food Research, 2018, 62(6):1700954.
[20] STRANDWITZ P,KIM K H,TEREKHOVA D, et al.GABA-modulating bacteria of the human gut microbiota[J].Nature Microbiology, 2019, 4(3):396-403.
[21] HWANG I, JO K, SHIN K C, et al.GABA-stimulated adipose-derived stem cells suppress subcutaneous adipose inflammation in obesity[J].Proceedings of the National Academy of Sciences of the United States of America, 2019, 116(24):11 936-11 945.
[22] PEDERSEN H K,GUDMUNDSDOTTIR V,NIELSEN H B, et al.Human gut microbes impact host serum metabolome and insulin sensitivity[J].Nature, 2016, 535(7 612):376-381.
[23] SONNER J K,KEIL M,FALK-PAULSEN M, et al.Dietary tryptophan links encephalogenicity of autoreactive T cells with gut microbial ecology[J].Nature Communications, 2019, 10(1):4877.
[24] SCOTT S A, FU J J, CHANG P V.Microbial tryptophan metabolites regulate gut barrier function via the aryl hydrocarbon receptor[J].Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(32):19 376-19 387.
[25] AGUS A, PLANCHAIS J, SOKOL H.Gut microbiota regulation of tryptophan metabolism in health and disease[J].Cell Host & Microbe, 2018, 23(6):716-724.
[26] DU J J, ZHANG P W, LUO J, et al.Dietary betaine prevents obesity through gut microbiota-drived microRNA-378a family[J].Gut Microbes, 2021, 13(1):1862612.
[27] REIMER R A.Establishing the role of diet in the microbiota–disease axis[J].Nature Reviews Gastroenterology & Hepatology, 2019, 16(2):86-87.
[28] BLACHER E, LEVY M, TATIROVSKY E, et al.Microbiome-modulated metabolites at the interface of host immunity[J].Journal of Immunology, 2017, 198(2):572-580.
[29] TURNBAUGH P J,LEY R E,MAHOWALD M A, et al.An obesity-associated gut microbiome with increased capacity for energy harvest[J].Nature, 2006, 444(7 122):1 027-1 031.
[30] MAGNE F, GOTTELAND M, GAUTHIER L, et al.The firmicutes/bacteroidetes ratio:A relevant marker of gut dysbiosis in obese patients?[J].Nutrients, 2020, 12(5):1474.
[31] KONG C, GAO R Y, YAN X B, et al.Probiotics improve gut microbiota dysbiosis in obese mice fed a high-fat or high-sucrose diet[J].Nutrition, 2019, 60:175-184.
[32] OLIVER L, RAMIÓ-PUJOL S, AMOEDO J, et al.A novel grape-derived prebiotic selectively enhances abundance and metabolic activity of butyrate-producing bacteria in faecal samples[J].Frontiers in Microbiology, 2021, 12:639948.
[33] CHEN H T, ZHANG F, ZHANG J, et al.A holistic view of berberine inhibiting intestinal carcinogenesis in conventional mice based on microbiome-metabolomics analysis[J].Frontiers in Immunology, 2020, 11:588079.
[34] LIU Z,DAI X,ZHANG H, et al.Gut microbiota mediates intermittent-fasting alleviation of diabetes-induced cognitive impairment [J].Nature Communications, 2020, 11(1):855.
[35] RIZVI Z A, DALAL R, SADHU S, et al.High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity[J].Science Advances, 2021, 7(37):eabg5016.
[36] PUJO J, PETITFILS C, LE FAOUDER P, et al.Bacteria-derived long chain fatty acid exhibits anti-inflammatory properties in colitis[J].Gut, 2021, 70(6):1 088-1 097.
[37] CEKANAVICIUTE E, YOO B B, RUNIA T F, et al.Gut bacteria from multiple sclerosis patients modulate human T cells and exacerbate symptoms in mouse models[J].Proceedings of the National Academy of Sciences of the United States of America, 2017, 114(40):10 713-10 718.
[38] ROM O, LIU Y H, LIU Z P, et al.Glycine-based treatment ameliorates NAFLD by modulating fatty acid oxidation, glutathione synthesis, and the gut microbiome[J].Science Translational Medicine, 2020, 12(572):eaaz2841.
[39] HUART J,LEENDERS J,TAMINIAU B, et al.Gut microbiota and fecal levels of short-chain fatty acids differ upon 24-hour blood pressure levels in men [J].Hypertension, 2019, 74(4):1 005-1 013.
[40] THINGHOLM L B,RÜHLEMANN M C,KOCH M, et al.Obese individuals with and without type 2 diabetes show different gut microbial functional capacity and composition [J].Cell Host Microbe, 2019, 26(2):252-264.
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