To study the effects of Bifidobacterium lactis XLTG11 on immune function of cyclophosphamide (CTX) induced immunosuppressed mice. BALB/c mice were randomly divided into seven groups (eight mice per group). Blank group and CTX group mice were given the same volume of saline water, and mice in levamisole hydrochloride group was administered 10 mg/kg levamisole hydrochloride. Mice in different groups received 2.5×106, 2.5×107, 2.5×108 or 2.5×109 CFU/mL B. lactis XLTG11 respectively. All mice were orally given once a day for 28 consecutive days. On 23rd day of administration, mice were intraperitoneally injected with 40 mg/kg CTX for two consecutive days, except for mice in the blank control group. On 28th day, the total white blood cell count and serum hemolysin level in seven groups of mice were observed. Meanwhile, the percentages of spleen lymphocyte subsets were analyzed by flow cytometry. The results showed that compare with blank group, CXT group had decreased white blood cell count, serum half hemolysis value (HC50), percentage of CD3＋, CD19＋, CD4＋CD8－ and CD4－CD8＋ in spleen lymphocytes (P<0.05). XLTG11 administration significantly increased HC50 value and enhanced the percentage of CD3＋,CD4＋CD8－ and CD4－CD8＋ in spleen lymphocytes in a dose dependent manner compared with CXT group (P<0.05). In summary, B. lactis XLTG11 can strengthen immune function.
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