Food and Fermentation Industries >

2022 , Vol. 48 >Issue 13: 108 - 114

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029849

Medium optimization for Chlorella sp. MBFJNU-17 heterotrophic cultivation

  • ZHOU Youcai ,
  • XIAO Xuehua ,
  • LIANG Zhibo ,
  • HE Yongjin ,
  • CHEN Bilian
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  • 1(College of Life Sciences, Fujian Normal University, Fuzhou 350117,China)
    2(Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350117, China)

Received date: 2021-10-27

  Revised date: 2021-11-18

  Online published: 2022-08-03

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Abstract

Urea was selected as the single nitrogen source for Chlorella sp. MBFJNU-17 heterotrophic cultivation. The results showed that the maximum biomass and the protein content of Chlorella was 10.85 g/L and 44.5% in batch cultivation using urea as nitrogen source. Meantime, the stable pH of cultures could benefit for the growth of Chlorella treated by urea. The significant factors that affected the growth of Chlorella were screened via Plackett-Burman experiments. Further, the concentrations of glucose, urea and trace elements stock solution were optimized by response surface methodology. And the biomass of Chlorella cultivated in the optimized medium was significantly increased to 15.53 g/L with the protein content of 52.3%. The essential amino acid index (EAAI) of Chlorella sp. MBFJNU-17 protein and soybean protein were compared with FAO/WHO/UNU standard. The results showed that the EAAI of Chlorella was 0.724 which higher than soybean protein (0.657). Taken together, Chlorella sp. MBFJNU-17 attained high density of biomass by the medium optimization, and exhibited more nutritional value than soybean protein, which can develop for animal feed or human food protein.

Key words： urea; Chlorella; response surface methodology; protein; essential amino acid

Cite this article

ZHOU Youcai , XIAO Xuehua , LIANG Zhibo , HE Yongjin , CHEN Bilian . Medium optimization for Chlorella sp. MBFJNU-17 heterotrophic cultivation[J]. Food and Fermentation Industries, 2022 , 48(13) : 108 -114 . DOI: 10.13995/j.cnki.11-1802/ts.029849

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