A method for the simultaneous determination of 37 veterinary residues in chicken by QuEChERS and UPLC-MS-MS was developed in this study. The sample was supersonically extracted with methanol-acetonitrile (2∶1) by DISQUETM extraction tube, the supernatant liquid was defatted with n-hexane, then dried under nitrogen stream and redissolved with acetonitrile-ammonium acetate (1∶9). The separation of the target objects was performed on the Waters Acquity UPLC BEH C18 column using methanol-0.05mmol/L ammonium acetate solution (0.1% formic acid) as a gradient elution. The identification and external standard method quantification were achieved by using electrospray ionization in positive ion mode (ESI+) with multiple reaction monitoring (MRM). The matrix-matched internal standard calibration curves were used for quantification determination. The linear range was from 1-50 μg/kg. The average recoveries and relative standard deviations were 71%-104% and 3.1%-14.7% respectively in the spiked range of 1-20 μg/kg. The limits of detection were 0.5 μg/kg, and the limits of quantification were 1 μg/kg for 37 veterinary residues. The method is rapid, simple, sensitive and accurate. It is suitable for the quantitative determination and confirmation of sulfonamide, quinolone, benzimidazole, tetracycline, macrolide and penicillin.
HUANG Hua
,
XIE Wendong
,
GU Yu
,
MENG Zhaoqi
,
SUN Yuanjie
,
DU Hongmiao
,
SUN Yana
,
KANG Shuai
. Determination of 37 veterinary residues in chicken by QuEChERS-UPLC-MS-MS[J]. Food and Fermentation Industries, 2022
, 48(13)
: 290
-295
.
DOI: 10.13995/j.cnki.11-1802/ts.029328
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