Food and Fermentation Industries >

2022 , Vol. 48 >Issue 13: 336 - 344

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031312

Research progress of β-glucosidase assay and its application

  • YIN Shouliang ,
  • YANG Yiying ,
  • XU Wen ,
  • ZHANG Yingchao ,
  • WANG Wenwen ,
  • LI Qiuyuan ,
  • ZHOU Zhijiang
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  • 1(School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China)
    2(School of Life Sciences, North China University of Science and Technology, Tangshan 063210, China)
    3(Jitang College of North China University of Science and Technology, Tangshan 063210, China)
    4(Postdoctoral Innovation Practice Base, Zhongrong Technology Co.,Ltd. Tangshan 064499, China)

Received date: 2022-02-27

  Revised date: 2022-03-21

  Online published: 2022-08-03

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Abstract

The enzyme β-glucosidase holds importance due to its commercial applications in food industries, biofuel and agricultural feeds, and also has a wide range of application in environmental monitoring assessments, medical diagnoses and other fields. Based on the types of substrates, the structure and characteristics of hydrolysis products catalyzed by β-glucosidase, the methods used for measuring enzymatic activities can be classified into spectrophotometry, fluorescence, electrochemistry and enzyme activity assay kits. Here, the working principle, advantages and disadvantages of each method are reviewed and discussed, the new methods for determination of β-glucosidase activity in living cells and the latest research progress in screening of β-glucosidase inhibitors are emphasized and elaborated. The main purpose of this review is to provide the method basis for promoting the application of β-glucosidase in the future.

Key words： β-glucosidase; living cells; inhibitors

Cite this article

YIN Shouliang , YANG Yiying , XU Wen , ZHANG Yingchao , WANG Wenwen , LI Qiuyuan , ZHOU Zhijiang . Research progress of β-glucosidase assay and its application[J]. Food and Fermentation Industries, 2022 , 48(13) : 336 -344 . DOI: 10.13995/j.cnki.11-1802/ts.031312

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