Food and Fermentation Industries >

2022 , Vol. 48 >Issue 14: 263 - 271

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031175

Determination of 70 kinds of pesticide residues in Kashi pomegranate by QuEChERS-gas chromatography-triple quadrupole mass spectrometry

  • YUMAIER Abulimiti ,
  • CAO Xu ,
  • CUI Zhichao ,
  • YUSUF Abdullah
Expand
  • 1(College of Chemistry and Environmental Science, Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry, Kashi University, Kashi 844000, China)
    2(Xinjiang Academy of Analysis and Testing, Urumqi 830000, China)

Received date: 2022-02-17

  Revised date: 2022-03-18

  Online published: 2022-08-19

Fold

Abstract

To establish a method for the simultaneous determination of 70 kinds of pesticide residues in three varieties of Kashi pomegranate by QuEChERS-gas chromatography-triple quadrupole mass spectrometry.Three varieties of Kashi pomegranate were extracted and purified by optimized QuEChERS procedure, gas-mass spectrometry conditions were established based on the characteristic fragment ions of 70 pesticide residues.The detection was carried out in multiple reaction monitoring mode, and the matrix matching external standard method was used for quantitative analysis.The results showed that the 70 kinds of pesticide residues had a good linear relationship in the range of 0-1.0 μg/kg, and the correlation coefficient was greater than 0.995.The limit of detection was 0.005-0.02 mg/kg. Totally 70 pesticide residues ranged from 76.4% to 108% in 0.01mg/kg; ranged from 75.4%-107%in 0.05 mg/kg; ranged from 71.6%-108%in 0.1mg/kg. The relative standard deviation (RSD) was 0.2%-11.2% (n=5) and the difference was statistically significant (P<0.05).This method is easy, simple, fast, efficient and has good repeatability and stability, which can be used for the quantitative detection and qualitative identification of pesticide residues in multi pigment sample such as Kashi pomegranate with high sugar content and large interference.

Key words： kashi pomegranate; QuEChERS; gas chromatography-triple quadrupole mass spectrometry; pesticide residues

Cite this article

YUMAIER Abulimiti , CAO Xu , CUI Zhichao , YUSUF Abdullah . Determination of 70 kinds of pesticide residues in Kashi pomegranate by QuEChERS-gas chromatography-triple quadrupole mass spectrometry[J]. Food and Fermentation Industries, 2022 , 48(14) : 263 -271 . DOI: 10.13995/j.cnki.11-1802/ts.031175

References

[1] TEIXEIRA DA SILVA J A, RANA T S, NARZARY D, et al.Pomegranate biology and biotechnology:A review[J].Scientia Horticulturae, 2013, 160:85-107.
[2] 贾晓辉, 张鑫楠, 刘艳, 等.石榴的营养、功效及应用[J].果树实用技术与信息, 2021(4):46-47.
JIA X H, ZHANG X N, LIU Y, et al.Nutrition, efficacy and application of pomegranate[J].Practical Techniques and Information on Fruit Trees, 2021(4):46-47.
[3] 梁智, 邹耀湘.新疆南疆石榴树平衡施肥技术试验研究[J].新疆农业科学, 2010, 47(2):345-350.
LIANG Z, ZOU Y X.Effect of balanced fertilization on pomegranate tree in southern area of Xinjiang[J].Xinjiang Agricultural Sciences, 2010, 47(2):345-350.
[4] 郝庆, 吴名武, 陈先荣.新疆石榴栽培与内地的差异[J].新疆农业科学, 2005,42(S1):41-44.
HAO Q, WU M W, CHEN X R.The difference of pomegranate cultivation between Xinjiang and the interior of China[J].Xinjiang Agricultural Sciences, 2005,42(S1):41-44.
[5] 薛晓珍. 新疆石榴的营养成分及用途[J].仪器仪表与分析监测, 2002(3):44-45.
XUE X Z.Nutritional components and uses of Xinjiang pomegranate[J].Instrumentation Analysis Monitoring, 2002(3):44-45.
[6] 刘文江. 新疆石榴(Punica L.)资源及其开发利用[J].干旱区研究, 2007,24(2):219-222.
LIU W J.Resources of Punica granatum Linn.and its exploitation and utilization in Xinjiang[J].Arid Zone Research,2007,24(2):219-222.
[7] 常占瑛, 刘桂花, 王梅, 等.新疆石榴皮HPLC指纹图谱研究[J].中国中医药信息杂志, 2019, 26(4):74-77.
CHANG Z Y, LIU G H, WANG M, et al.Study on HPLC fingerprints of granati pericarpium[J].Chinese Journal of Information on Traditianal Chinese Medicine, 2019, 26(4):74-77.
[8] 孟新涛, 潘俨, 许铭强, 等.随机质心映射优化法(RCO)提取皮亚曼石榴皮中黄酮类化合物[J].食品工业科技, 2016, 37(2):276-280;286.
MENG X T, PAN Y, XU M Q, et al.Random centroid optimization of flavonoids extraction from Pierman pomegranate[J].Science and Technology of Food Industry, 2016, 37(2):276-280;286.
[9] 陈芸, 詹建立, 阿依西古丽·外力, 等.南疆石榴主栽品种调查及果实品质初步鉴定[J].黑龙江农业科学, 2016(1):83-86.
CHEN Y, ZHAN J L, WALI A, et al.Main cultivars investigation and fruit quality identification of Punica granatum L.in south Xinjiang[J].Heilongjiang Agricultural Sciences, 2016(1):83-86.
[10] 尹燕雷, 任建辉, 何天明, 等.新疆的石榴栽培状况[J].落叶果树, 2006,38(6):15-16.
YIN Y L, REN J H, HE T M, et al.Culture status of pomegranate in Xinjiang[J].Deciduous Fruits, 2006,38(6):15-16.
[11] 买合木提·买买提, 热比耶姆·毛拉托合提, 吐逊古丽·撒塔尔, 等.传统维药石榴皮的化学成分及药理作用研究进展[J].中国民族医药杂志, 2017, 23(1):54-57.
MAIHEMUTI M, MAIBIYEMU M, TUSUNGULI S, et al.Research progress on chemical constituents and pharmacological effects of traditional uygur medicine pomegranate peel[J].Journal of Medicine and Pharmacy of Chinese Minorities, 2017, 23(1):54-57.
[12] 刘宇, 蔡霞, 曾勇, 等.石榴药理研究新进展[J].世界科学技术-中医药现代化, 2015, 17(3):679-686.
LIU Y, CAI X, ZENG Y, et al.New development on pharmacological effects of Punica granatum L.[J].Modernization of Traditional Chinese Medicine and World Science and Technology, 2015, 17(3):679-686.
[13] DAHANE S, GIL GARCíA M D, UCLÉS MORENO A, et al.Determination of eight pesticides of varying polarity in surface waters using solid phase extraction with multiwalled carbon nanotubes and liquid chromatography-linear ion trap mass spectrometry[J].Microchimica Acta,2015, 182(1-2):95-103.
[14] MNYANDU H M, MAHLAMBI P N.Optimization and application of QuEChERS and SPE methods followed by LC-PDA for the determination of triazines residues in fruits and vegetables from Pietermaritzburg local supermarkets[J].Food Chemistry, 2021, 360:129818.
[15] ZHOU Q Z, LIU Z Q, LIU F M, et al.Determination of desmedipham residue in 21 foods by HPLC-MS/MS combined with a modified QuEChERS and mixed-mode SPE clean-up method[J].Journal of Food Composition and Analysis, 2021, 102:104004.
[16] JADHAV M, THEKKUMPURATH A S, NAKADE M, et al.Multiresidue method for targeted screening of pesticide residues in spice cardamom (Elettaria cardamomum) by liquid chromatography with tandem mass spectrometry[J].Journal of AOAC INTERNATIONAL, 2019, 100(3):603-609.
[17] NAVICKIENE S, SANTOS L F S,DOS REISSILVA A, et al.Use of magnesium silicate as a new type of adsorbent for dispersive solid-phase extraction cleanup of the quick, cheap, effective, rugged, and safe method for pesticides during analysis of lager beer by gas chromatography-tandem mass spectrometry[J].Journal of AOAC INTERNATIONAL, 2019, 102(2):619-624.
[18] 张艳峰, 徐鹏, 王会利.QuEChERS技术结合气相色谱-串联质谱法筛查花椒中115种农药残留[J].农药学学报, 2020, 22(3):493-503.
ZHANG Y F, XU P, WANG H L.Screening of 115 pesticide residues in Chinese prickly ash by QuEChERS-gas chromatography-tandem mass spectrometry[J].Chinese Journal of Pesticide Science, 2020, 22(3):493-503.
[19] 徐炎炎, 李森, 张芹, 等.气质联用和液质联用中基质效应的分析和总结[J].农药, 2017, 56(3):162-167.
XU Y Y, LI S, ZHANG Q, et al.Analysis and summary of matrix effects in GC-MS and LC-MS[J].Agrochemicals, 2017, 56(3):162-167.
[20] 熊颖, 李纯, 侯惠婵, 等.QuEChERS-GC-MS/MS测定化橘红65种农药残留[J].中国现代中药, 2021, 23(6):1 043-1 050.
XIONG Y, LI C, HOU H C, et al.Determination of 65 pesticide residues in citri grandis exocarpium by QuEChERS-GC-MS/MS[J].Modern Chinese Medicine, 2021, 23(6):1 043-1 050.
[21] 鲍忠赞. 气相色谱法探究果蔬中有机氯和拟除虫菊酯类农药残留的基质效应[J].农药, 2018, 57(12):890-894.
BAO Z Z.Matrix effects of organochlorine and pyrethroid pesticides in fruits and vegetables using gas chromatography[J].Agrochemicals, 2018, 57(12):890-894.
[22] 国家卫生健康委员会,农业农村部,国家市场监督管理总局. 食品安全国家标准 植物源性食品中208种农药及其代谢物残留量的测定 气相色谱-质谱联用法:GB 23200.113—2018[S].北京:中国标准出版社, 2019.
National Health Commission of the People's Republic of China,Ministry of Agriculture and Rural Affairs of the People's Republic of China, State Administration of Market Regulation.GB 23200.113—2018 National food safety standard determination of 208 pesticides and their metabolites residues in plant-derived foods gas chromatography-mass spectrometry[S].Beijing:Standards Press of China, 2019.
[23] 杨芳. QuEChERS联合GC-MS/MS和LC-MS/MS建立生姜及其制品中88种农药残留的检测方法及应用[D].南昌:江西农业大学, 2018.
YANG F.The detection method and application of 88 peslticide residues of ginger and its products established by QuEChERS-GC-MS/MS and QuEChERS-LC-MS/MS[D].Nanchang:Jiangxi Agricultural University, 2018.
[24] 汪霞, 滕岳臻.气质联用法测定蔬菜中44种农药多残留的QuECHERS方法研究[J].中国医药指南, 2017, 15(14):30-32.
WANG X, TENG Y Z.QuECHERS method for determination of 44 pesticide residues in vegetables by GC-MS[J].Guide of China Medicine, 2017, 15(14):30-32.
Options
Outlines

/

〈 〉

Powered by Beijing Magtech Co. Ltd,.