In beer industry, the flocculation properties of Saccharomyces pastorianus is one of the important factors affecting the production of beer. In the application of Saccharomyces pastorianus L-1 in large-scale production, problems such as short yeast suspension stability period and high mortality rate of reused yeast mud existed, resulting in incomplete fermentation, thus affecting the reduction of undesirable flavor substances and the final beer flavor quality. Therefore, L-1 was used as the starting strain to perform atmospheric and room temperature plasma (ARTP) mutagenesis and the mutagenic strains were screened for the first and second screening. Three target strains 179, 293, and 361 with weakened flocculation and good genetic stability were obtained. The flocculation capacity was reduced by 6.67%, 6.67%, and 5.56% respectively, the mortality rate was reduced by 31.21%, 34.57%, and 16.93% respectively, and the corresponding acetaldehyde content in the fermentation broth was reduced by 19.53%, 19.17%, and 15.83%. Indexes such as carbon source consumption, α-amino nitrogen, and alcohol production capacity had all been improved, and the content of major higher alcohols has not changed significantly. The acquisition of these strains has certain practical application potential in the production of beer industry.
LIU Xiaohang
,
RUAN Yulei
,
SU Meng
,
FU Xiaofen
,
HE Xiuli
,
GUO Liyun
,
WU Xiaole
,
GUO Xuewu
,
XIAO Dongguang
,
CHEN Yefu
. Breeding of Saccharomyces pastorianus with weak flocculation[J]. Food and Fermentation Industries, 2022
, 48(15)
: 47
-54
.
DOI: 10.13995/j.cnki.11-1802/ts.030107
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