In order to identify the main groups of endophytic fungi and pathogen in Crison grape fruits during storage, morphological and molecular biological methods were used to determine the classification status of the strains, also their spoilage ability on different fruits were determined. The results showed that the endophytic strain K1-K13 from healthy Crison fruit showed the same culture characteristics on PDA medium. The colony whitish, flat, with floccose mycelium, which was consistent with the morphological characteristics of Acremonium. ITS and 28S rDNA D1/D2 region sequences were used to construct an evolutionary tree analysis, and it clustered with Acremonium sclerotigenum, so it was identified as A. sclerotigenum. Inoculation of A. sclerotigenum on healthy grapes, tomatoes, and apples, caused rot, which indicated its spoilage ability. At the same time, Botrytis cinerea and Alternaria sp. were isolated from rotting Crison grape fruits. A. sclerotigenum strains had no antagonistic effect on B. cinerea or Alternaria sp. In this study, a pathogenic endophyte from Crison fruits was reported for the first time, which enriched the resources of grape endophytes and provided technical and theoretical support for the control of grape postharvest diseases.
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