Food and Fermentation Industries >

2022 , Vol. 48 >Issue 16: 50 - 55

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029010

Establishment of an electrochemical immunosensor for marbofloxacin analysis based on graphene oxide-chitosan nanocomposite film modified glass carbon electrode

  • QIU Caixia ,
  • CHEN Xiujin ,
  • FU Zuyao ,
  • LI Zhaozhou ,
  • WANG Yao ,
  • DONG Yifan ,
  • SUN Fengxia ,
  • GAO Dong
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China)
    2(National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471000, China)
    3(School of Food Science and Technology, Shihezi University, Shihezi 832003, China)

Received date: 2021-08-13

  Revised date: 2021-10-11

  Online published: 2022-09-16

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Abstract

A reduced graphene oxide-chitosan nanocomposite film modified the glassy carbon electrode was prepared using electrochemical reduction of graphene oxide. And then the antibody against marbofloxacin was immobilized on the surface of the modified electrode to construct an electrochemical immunosensor for highly sensitive detection of marbofloxacin. The surface morphology of graphene oxide and nanocomposite film were characterized using a scanning electron microscope. The detection conditions of the electrochemical immunosensor for the determination of marbofloxacin were optimized with cyclic voltammetry and differential pulse voltammetry. Under optimal conditions, the peak currents of differential pulse voltammetry of immunosensor were proportional to the concentration of marbofloxacin in the range of 2.0-40.0 ng/mL with a detection limit of 0.08 ng/mL. Recovery experiments were performed using artificially contaminated pork samples and milk samples, and recovery rates reached 84.4%-96.36%. As a result, the established immunosensor was accurate, stable and sensitive for rapid detection of marbofloxacin in animal-derived foods.

Key words： graphene oxide; chitosan; marbofloxacin; electrochemical immunosensor; differential pulse voltammetry

Cite this article

QIU Caixia , CHEN Xiujin , FU Zuyao , LI Zhaozhou , WANG Yao , DONG Yifan , SUN Fengxia , GAO Dong . Establishment of an electrochemical immunosensor for marbofloxacin analysis based on graphene oxide-chitosan nanocomposite film modified glass carbon electrode[J]. Food and Fermentation Industries, 2022 , 48(16) : 50 -55 . DOI: 10.13995/j.cnki.11-1802/ts.029010

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