Food and Fermentation Industries >

2022 , Vol. 48 >Issue 16: 56 - 64

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030228

Breeding and fermentation optimization of high titer pyrrorole-quinoline quinone strain

  • ZHANG Jing ,
  • LIU Mengsu ,
  • QIN Zhijie ,
  • ZENG Weizhu ,
  • ZHOU Jingwen
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  • 1(National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)
    2(Science Center for Future Foods, Jiangnan University, Wuxi 214122, China

Received date: 2021-11-26

  Revised date: 2021-12-28

  Online published: 2022-09-16

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Abstract

Pyrroloquinoline quinone (PQQ) is a reddish-brown aromatic tricyclic ortho-quinone compound that functions as an electron transport cofactor. PQQ has high application value in food, medicine and cosmetics industries. In recent years, the production of PQQ by microbial fermentation methods has gained considerable interest. In this study, Hyphomicrobium denitrificans (CGMCC 1.12893) was selected as the starting strain, which could produce 31.4 mg/L PQQ at the shake flask scale. Through the combination of ARTP mutation and high-throughput screening method, a high-yielding mutant strain of PQQ was screened and numbered as 34, which accumulated 48.4 mg/L of PQQ, a 54.1% increase over the starting strain. For this strain, the components in the shake flask fermentation medium, including carbon source, nitrogen source, metal ions and vitamin were optimized, and the yield of PQQ increased to 105.2 mg/L. Finally, the production of PQQ was further increased to 349.8 mg/L in a 5 L bioreactor. In conclusion, the PQQ high-producing mutant strain was obtained through random mutagenesis screening, and production of PQQ was further improved through medium optimization in shake flask and fermentation in a 5 L bioreactor. The results laid a groundwork for the subsequent development of fermentation process control strategies.

Key words： Hyphomicrobium denitrificans; pyrrorolequinoline quinone; atmospheric and room temperature plasma (ARTP) mutagenesis; high-throughput screening; fermentation optimization

Cite this article

ZHANG Jing , LIU Mengsu , QIN Zhijie , ZENG Weizhu , ZHOU Jingwen . Breeding and fermentation optimization of high titer pyrrorole-quinoline quinone strain[J]. Food and Fermentation Industries, 2022 , 48(16) : 56 -64 . DOI: 10.13995/j.cnki.11-1802/ts.030228

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