Purification of recombinant Marsupenaeus japonicus ferritin by saturated ammonium sulfate

  • MA Guihong ,
  • LAI Longxin ,
  • LI Die ,
  • FENG Yinglin ,
  • LI Xiao ,
  • ZHANG Zhiqing ,
  • LI Shuhong ,
  • WANG Caixia ,
  • GU Yi ,
  • MA Yi ,
  • LI Meiliang
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  • 1(College of Food Science, Sichuan Agricultural University, Ya′an 625014, China)
    2(Mianyang Zhonggou Ecological Agriculture Technology Co.Ltd., Mianyang 621000, China)
    3(Sichuan Runzhao Fishery Co.Ltd., Ya′an 625000, China)

Received date: 2021-09-08

  Revised date: 2021-10-11

  Online published: 2022-09-16

Abstract

At present, recombinant ferritin is widely used as functional nanomaterial. Firstly, after heat treatment (65 ℃, 10 min), the crude extract of recombinant Marsupenaeus japonicus ferritin (rMjF) was treated with 20% saturated ammonium sulfate. Secondly, the saturation of ammonium sulfate in the treated supernatant increased to 40%. Then, the precipitate was dissolved in buffer again. Finally, rMjF was successfully purified by non-column chromatography such as dialysis and ultrafiltration. The results showed that the crude extract of rMjF after heating treatment was treated with saturated ammonium sulfate compound treatment, the content of hybrid protein greatly reduced. The rMjF was apoferritin with only one subunit (about 20 kDa), with an outer diameter of about 12 nm. Fourier transform infrared spectroscopy analysis showed that the highest ɑ-helix relative content in rMjF was 57.91%. This method is convenient for mass separation and purification of rMjF, and provides a certain reference value for the study of rMjF as a nano transport carrier.

Cite this article

MA Guihong , LAI Longxin , LI Die , FENG Yinglin , LI Xiao , ZHANG Zhiqing , LI Shuhong , WANG Caixia , GU Yi , MA Yi , LI Meiliang . Purification of recombinant Marsupenaeus japonicus ferritin by saturated ammonium sulfate[J]. Food and Fermentation Industries, 2022 , 48(16) : 138 -143 . DOI: 10.13995/j.cnki.11-1802/ts.029329

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