To test the effects of Lactobacillus plantarum P-8 on the intestinal flora, antioxidant and anti-inflammatory capacity of mice for providing a basis for the development and utilization of a probiotic preparation of L. plantarum P-8, Fifty-two BALB/c male mice were randomly divided into blank control, low, medium and high-dose groups and administered with normal saline and different doses of probiotic preparation of L. plantarum P-8 for 30 d. The results showed that, compared with the control, there was no significant difference on the food intake, body weight and organ coefficient in each dose group(P>0.05), the levels of GSH, T-SOD and GSH-Px in the small intestine and liver tissues significantly increased (P<0.05) and the levels of MDA significantly decreased in the high-dose group (P<0.05) and the levels of GSH-Px significantly increased in the medium-dose group (P<0.05). The levels of IL-1β and IL-6 significantly decreased in the high-dose group (P<0.05). After administration, there was no significant difference in the number of microorganisms in blank control, low and medium-dose groups (P>0.05), the number of Lactobacillus and Bifidobacterium significantly increased in the high-dose group (P>0.05). 16S rDNA high-throughput sequencing results showed that, compared with the control, the abundance of Lactobacillus significantly increased in the low-dose group (P<0.05) and extremely increased in the medium and high-dose group (P<0.05), the abundance of Odoribacter significantly increased (P<0.05) and that of Muribaculum and Bacillus decreased in the high-dose group significantly (P<0.05). In summary, L. plantarum P-8 can improve the antioxidant capacity, reduce serum inflammation level, regulate the composition of intestinal flora and has a great application potential in the development of probiotic preparation.
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