Food and Fermentation Industries >

2022 , Vol. 48 >Issue 17: 71 - 78

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030314

Genome mining, expression and activity determination of a novel antimicrobial peptide from Bacillus coagulans CGMCC 9951

  • ZHANG Jing ,
  • WU Ying ,
  • GU Shaobin ,
  • MA Jinliang ,
  • TIAN Pingping ,
  • ZHAO Lina ,
  • LI Xin ,
  • ZHANG Jie
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  • 1(Henan University of Science and Technology, Luoyang 471023, China)
    2(Henan Engineering Research Center of Food Microbiology, Luoyang 471023, China)

Received date: 2021-12-03

  Revised date: 2021-12-29

  Online published: 2022-10-01

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Abstract

Antimicrobial peptides are natural biological preservatives that inhibit the growth of food-borne pathogens. In order to obtain antimicrobial peptide from Bacillus coagulans CGMCC 9951, whole genome sequencing and bioinformatics analysis were used to mine the antimicrobial peptide synthesis gene cluster, and their expression and activity were verified. In order to study the antibacterial activity of the peptide, the fusion protein containing glutathione S-transferase (GST) tag was successfully constructed in Escherichia coli and verified by mass spectrometry. The results showed that two antimicrobial peptides were excavated by antiSMASH software. After comparison with National Center for Biotechnology Information (NCBI) database, Region 3 was found to be greatly similar with Amylocyclicin, and was selected for expression. It was found that mature Region 3 was composed of 64 amino acid residues with a molecular weight of 6 371.57 Da, which was positively hydrophobic protein. Homology of Region 3 showed cyclic antimicrobial peptide, and the tertiary structure was mainly α helix structure. Using Listeria monocytogenes as an indicator bacterium, the inhibition zone diameter was (15.2±1.4) mm, which confirmed that the expressed fusion protein had antibacterial activity. The successful expression of the fusion protein lays a foundation for the further study of B. coagulans CGMCC 9951 antimicrobial peptide.

Key words： Bacillus coagulans CGMCC 9951; whole genome sequencing; cyclic peptide; expression; antimicrobial activity

Cite this article

ZHANG Jing , WU Ying , GU Shaobin , MA Jinliang , TIAN Pingping , ZHAO Lina , LI Xin , ZHANG Jie . Genome mining, expression and activity determination of a novel antimicrobial peptide from Bacillus coagulans CGMCC 9951[J]. Food and Fermentation Industries, 2022 , 48(17) : 71 -78 . DOI: 10.13995/j.cnki.11-1802/ts.030314

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