Food and Fermentation Industries >

2022 , Vol. 48 >Issue 17: 122 - 128

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028616

Bacterial community structure and lactic acid bacterial taxa in Zha-chili from Tianmen region

  • XI La ,
  • XIANG Fanshu ,
  • ZHANG Yan ,
  • ZHANG Haibo ,
  • GUO Zhuang
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  • 1(Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang 441053, China)
    2(Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory, Hubei University of Arts and Science, Xiangyang 441053, China)
    3(Research Institute of Biotechnology, Angel Yeast Co.Ltd., Yichang 443003, China)

Received date: 2021-07-10

  Revised date: 2022-01-14

  Online published: 2022-10-01

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Abstract

The bacterial community structure and function of Zha-chili from Tianmen region were analyzed by MiSeq high-throughput sequencing and PICRUSt software, respectively, and the lactic acid bacteria were isolated, identified and preserved by a pure culture method. Fifteen samples were divided into two clusters. The results showed that the bacteria in cluster I were mainly affiliated to Lactobacillus, with a relative abundance of 89.77%; the bacteria in cluster II were mainly affiliated to Klebsiella, Kosakonia, and Tetragenococcus, with relative abundances of 19.81%, 12.24%, and 10.89%, respectively. The two clusters were found to be highly significantly different (P<0.001) from one another by Mann Whitney test; meanwhile, a significant negative correlation observed between Lactobacillus and other bacterial genera such as Kosakonia and Pseudomonas (P<0.05). There were significant differences in 15 functions among the two clusters, but both of them were highly expressed in the transport and metabolism functions of amino acids and carbohydrates. The results of isolation and identification of lactic acid bacteria showed that L. plantarum, L. brevis, L. curvatus and L. sakei were contained in Zha-chili, and L. plantarum was the dominant Lactobacillus in Zha-chili from the Tianmen region.

Key words： MiSeq high-throughput sequencing; Zha-chili; bacterial diversity; functional prediction; lactic acid bacteria

Cite this article

XI La , XIANG Fanshu , ZHANG Yan , ZHANG Haibo , GUO Zhuang . Bacterial community structure and lactic acid bacterial taxa in Zha-chili from Tianmen region[J]. Food and Fermentation Industries, 2022 , 48(17) : 122 -128 . DOI: 10.13995/j.cnki.11-1802/ts.028616

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