Analysis of characteristics in volatile component of Gujinggong Baijiu with different aging times based on HS-SPME-GC×GC-TOFMS

  • LING Yuting ,
  • CHEN Shuang ,
  • XU Yan ,
  • FAN Wenlai
Expand
  • 1(School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(Lab of Brewing Microbiology and Applied Enzymology, Jiangnan University, Wuxi 214122, China)
    3(Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China)

Received date: 2022-03-10

  Revised date: 2022-03-25

  Online published: 2022-10-01

Abstract

The volatile compounds in Gujinggong Baijiu with different aging times were characterized by headspace solid phase microextraction(HS-SPME) with comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry(GC×GC-TOFMS). Through the identification strategy, 262 potential aroma active components were accurately identified. In addition to alcohol, aldehyde, acid, and ester compounds, a variety of trace aroma compounds in Gujinggong Baijiu were richest. 50 substances with significant differences in the content of Gujinggong Baijiu at different aging times were analyzed by multivariate statistical analysis method. Most of the furan, terpene, aromatic and acid flavor compounds gradually increased with aging time, which contributed to the aroma of Baijiu samples. Chemometrics methods were used to analyze the Baijiu samples with different grades and aging times. The results showed that the differences of grades among Baijiu samples were more important than the differences of years, which confirmed that the quality control of Baijiu had certain significance for regulating the flavor quality of Baijiu aging.

Cite this article

LING Yuting , CHEN Shuang , XU Yan , FAN Wenlai . Analysis of characteristics in volatile component of Gujinggong Baijiu with different aging times based on HS-SPME-GC×GC-TOFMS[J]. Food and Fermentation Industries, 2022 , 48(17) : 241 -248 . DOI: 10.13995/j.cnki.11-1802/ts.031416

References

[1] 沈怡方. 白酒的香型、风格与流派[J].酿酒, 2003, 30(1):1-2.
SHEN Y F.The flavor type, style and school of liquor[J].Liquor Making, 2003, 30(1):1-2.
[2] 张杰, 程伟, 潘天全, 等.浓香型白酒风味成分研究现状及展望[J].酿酒, 2019, 46(1):29-32.
ZHANG J, CHENG W, PAN T Q, et al.Research progress about flavor substances of strong flavour Chinese spirits[J].Liquor Making, 2019, 46(1):29-32.
[3] 熊子书. 中国白酒贮存老熟的研究[J].酿酒科技, 2000(3):27-29;34.
XIONG Z S.Investigation on aging of stored liquor[J].Liquor-Making Science & Technology, 2000(3):27-29;34.
[4] 宋瑞滨, 孙琪, 梁成彬.浅谈浓香型白酒贮存及利用[J].酿酒科技, 2002(3):43-44.
SONG R B, SUN Q, LIANG C B.Discussion on storage of Luzhou-flavor liquor and its application[J].Liquor-Making Science & Technology, 2002(3):43-44.
[5] 胡志平. 浓香型白酒在陈酿过程中的酒质变化[J].酿酒科技, 2005(12):35-37;40.
HU Z P.Quality changes of Luzhou-flavor liquor during storage[J].Liquor-Making Science & Technology, 2005(12):35-37;40.
[6] 李家民. 浓香型白酒贮存过程中质量变化规律的研究及应用[J].酿酒, 2007, 34(4):110-116.
LI J M.Studies and applications on quality change rule during storage of Luzhou-flavor liquor[J].Liquor Making, 2007, 34(4):110-116.
[7] 仝建波, 陈洋, 程芳玲, 等.浓香型白酒贮存过程香味成分变化规律的研究[J].酿酒科技, 2010(10):17-18;22.
TONG J B, CHEN Y, CHENG F L, et al.Research on the change rules of flavoring compositions of Luzhou-flavor liquor during the storage[J].Liquor-Making Science & Technology, 2010(10):17-18;22.
[8] 张崇军. 浓香型白酒贮酒过程中酯、酸变化规律研究[J].食品工业, 2013, 34(1):147-150.
ZHANG C J.Content change of esters and acids during the storage of Luzhou-flavor liquor[J].The Food Industry, 2013, 34(1):147-150.
[9] 李冰川, 张强, 王开国, 等.浓香型白酒贮存过程中的酸酯动态平衡研究[J].酿酒科技, 2018(12):92-96.
LI B C, ZHANG Q, WANG K G, et al.Dynamic balance of acids and esters during the storage period of Nongxiang Baijiu[J].Liquor-Making Science & Technology, 2018(12):92-96.
[10] 曹玉发, 孙怡辰, 魏轩, 等.3个年份浓香型白酒香气成分比较研究[J].食品安全质量检测学报, 2021, 12(2):769-779.
CAO Y F, SUN Y C, WEI X, et al.Investigation on the aroma compounds of strong-aroma-type Baijiu in 3 different aging years[J].Journal of Food Safety & Quality, 2021, 12(2):769-779.
[11] FAN W L, QIAN M C.Headspace solid phase microextraction and gas chromatography-olfactometry dilution analysis of young and aged Chinese “Yanghe Daqu” liquors [J].Journal of Agricultural and Food Chemistry, 2005, 53(20):7 931-7 938.
[12] FAN W L, QIAN M C.Identification of aroma compounds in Chinese “Yanghe Daqu” liquor by normal phase chromatography fractionation followed by gas chromatography olfactometry[J].Flavour and Fragrance Journal, 2006, 21(2):333-342.
[13] 柳军. 口子窖和剑南春白酒香气物质研究[D].无锡:江南大学, 2008.
LIU J.Studies on aroma compounds of Chinese Kouzijiao and Jiannanchun liquors[D].Wuxi:Jiangnan University, 2008.
[14] NIU Y W, KONG J L, XIAO Z B, et al.Characterization of odor-active compounds of various Chinese “Wuliangye” liquors by gas chromatography-olfactometry, gas chromatography-mass spectrometry and sensory evaluation[J].International Journal of Food Properties, 2017, 20(sup1):S735-S745.
[15] 张伟建, 范文来, 徐岩, 等.沂蒙老区浓香型白酒香气成分分析[J].食品与发酵工业, 2019, 45(10):188-193.
ZHANG W J, FAN W L, XU Y, et al.Volatile aroma components in strong-aroma liquors produced in Yimeng old district[J].Food and Fermentation Industries, 2019, 45(10):188-193.
[16] LIU H L, SUN B G.Effect of fermentation processing on the flavor of baijiu[J].Journal of Agricultural and Food Chemistry, 2018, 66(22):5 425-5 432.
[17] MURRAY J A.Qualitative and quantitative approaches in comprehensive two-dimensional gas chromatography[J].Journal of Chromatography A, 2012, 1261:58-68.
[18] 刘志鹏. 全二维气相色谱—飞行时间质谱技术在白酒挥发性风味组分定性、定量分析中的应用[D].无锡:江南大学, 2019.
LIU Z P.The application of comprehensive two-dimensional gas chromatography/time of flight mass spectrometry in the qualitative and quantitative analysis of volatile flavor components in Chinese liquor[D].Wuxi:Jiangnan University, 2019.
[19] YAN Y, CHEN S, NIE Y, et al.Characterization of volatile sulfur compounds in soy sauce aroma type Baijiu and changes during fermentation by GC × GC-TOFMS, organoleptic impact evaluation, and multivariate data analysis[J].Food Research International, 2020, 131:109043.
[20] STEFANUTO P H, PERRAULT K A, DUBOIS L M, et al.Advanced method optimization for volatile aroma profiling of beer using two-dimensional gas chromatography time-of-flight mass spectrometry[J].Journal of Chromatography A, 2017, 1507:45-52.
[21] 高梦昕, 陈双, 刘志鹏, 等.中国白酒挥发性风味数据库的构建与应用[C] //2019年第四届中国白酒学术研讨会论文集.北京:中国轻工业出版社, 2019:348-360.
GAO M X, CHEN S, LIU Z P, et al.The development and application of Chinese Baijiu flavor library[C] //The 4th Baijiu Technology Symposium.Beijing:China Light Industry Press, 2019:348-360.
[22] WELDEGERGIS B T, CROUCH A M, GóRECKI T, et al.Solid phase extraction in combination with comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry for the detailed investigation of volatiles in South African red wines[J].Analytica Chimica Acta,2011,701(1):98-111.
[23] ZHAO D R, SHI D M, SUN J Y, et al.Characterization of key aroma compounds in Gujinggong Chinese Baijiu by gas chromatography-olfactometry, quantitative measurements, and sensory evaluation[J].Food Research International, 2018, 105:616-627.
[24] VAN GEMERT L J.Odour Thresholds:Compilations of Odour Threshold Values in Air, Water, and Other Media, 2nd ed[M].Utrechts:Oliemans Punter & Partners BV, 2011.
[25] WANG L, HU G Y, LEI L B, et al.Identification and aroma impact of volatile terpenes in Moutai liquor[J].International Journal of Food Properties, 2016, 19(6):1 335-1 352.
[26] 王志坚. 啤酒中醛类物质的形成[J].食品工业, 2004, 25(4):7-8.
WANG Z J.The formation of aldehydes in beer[J].The Food Industry, 2004,25 (4):7-8.
[27] ALAÑÓN M E, RUBIO H, DÍAZ-MAROTO M C, et al.Monosaccharide anhydrides, new markers of toasted oak wood used for ageing wines and distillates[J].Food Chemistry, 2010, 119(2):505-512.
Outlines

/