Molecular level strategy for high expression of foreign protein in Pichia pastoris

ZHANG Xinran; LING Yan; YANG Ying

doi:10.13995/j.cnki.11-1802/ts.029940

Food and Fermentation Industries >

2022 , Vol. 48 >Issue 17: 321 - 328

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029940

Molecular level strategy for high expression of foreign protein in Pichia pastoris

ZHANG Xinran ,
LING Yan ,
YANG Ying

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1(Institute of Radiation Medicine,Academy of Military Medical Science, Beijing 100850, China)
2(School of Life Sciences, Hebei University, Baoding 071000, China)

Received date: 2021-11-10

Revised date: 2022-01-05

Online published: 2022-10-01

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Abstract

Pichia pastoris expression system has many excellent characteristics and is one of the most successful foreign protein expression systems. Through the rapid development in recent decades, it has attracted wide attention from all walks of life, and has produced huge economic and social value. Compared with other existing expression systems, P. pastoris has great advantages in post-translational modification of target proteins, and has been widely used to express exogenous proteins. However, on the other hand, the system is affected by many factors, including the characteristics of exogenous genes, bacterial species, expression environment and fermentation technology, so it also shows great differences in the expression of different exogenous proteins. Gene transcription module, this paper focuses on the molecular level of protein secretion and degradation of modules and transport four modules, and introduces the use of P. pastoris expression system by optimizing codon, high copy number exogenous gene screening, gene knockout protease, and guide the peptides were folded factors to improve the efficiency of heterologous protein expression of related policies, The development prospect of the system is briefly prospected.

Key words： Pichia pastoris; yeast expression system; efficient expression; molecular level; foreign proteins

Cite this article

ZHANG Xinran , LING Yan , YANG Ying . Molecular level strategy for high expression of foreign protein in Pichia pastoris[J]. Food and Fermentation Industries, 2022 , 48(17) : 321 -328 . DOI: 10.13995/j.cnki.11-1802/ts.029940

References

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