Huangjiu is endowed with plentiful oligosaccharides in the low-temperature and long-term fermentation by a unique variety of microorganisms. In this study, Huangjiu oligosaccharides were isolated and purified from semi-dry Shaoxing Huangjiu. The oligosaccharides were extracted with 75% ethanol precipitation and Sevage method was applied to remove free proteins. Crude Huangjiu oligosaccharides were separated with 300 Da and 3000 Da membranes, subsequently freeze-drying was performed to obtain Huangjiu oligosaccharide powder, during which the extraction rate reached 31.80%. A highly-purified oligosaccharide HJO-a (purity of 84.21%) was obtained by DEAE Sepharose Fast Flow and Sephadex G15 column chromatography. An in vitro fermentation model of mouse feces was established to explore the probiotic effect of HJO-a on gut microbiota. The results showed that Huangjiu oligosaccharides were fermented by gut microbiota, which resulted in the increase of the acetic acid, propionic acid, butyric acid and isobutyric acid content, decreasing the pH of fermentation broth. Furthermore, Huangjiu oligosaccharides significantly increased the abundance of Bifidobacterium, Lactobacillus and Bacteroides (P<0.05), and evidently inhibited the proliferation of Escherichia coli in the fermentation broth (P<0.05). The aforementioned results revealed that Huangjiu oligosaccharides could exert potential prebiotic effects. The study provides a certain theoretical basis for the separation, purification, and functional activity analysis of Huangjiu oligosaccharides.
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