D-psicose 3-epimerase can catalyze the conversion of D-fructose to the rare sugar D-psicose, which is the key enzyme in the production of D-psicose. In order to increase the expression level of D-psicose 3-epimerase, B. subtilis WB800 was used as the host strain to heterologously express D-psicose 3-epimerase from Clostridium scindens ATCC 35704. First, recombinant strains with different single promoters and tandem promoters were successfully obtained. Through culturing in shaking flasks, the recombinant strains with the single promoter Phag showed the highest enzyme activity (approximately 19.62 U/mL), which was 1.3 times that of the original strain with P43. Then the Phag′s ribosome binding site sequence was mutated, and the enzyme activity of the mutant strain was further increased by 29.4%, which was 1.69 times that of the original strain. The research results provide a methodological reference for the industrial production of D-psicose 3-epimerase.
HU Mengying
,
LI Mengli
,
JIANG Bo
,
ZHANG Tao
. Expression of D-psicose 3-epimerase in Bacillus subtilis[J]. Food and Fermentation Industries, 2022
, 48(18)
: 42
-47
.
DOI: 10.13995/j.cnki.11-1802/ts.030501
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