Food and Fermentation Industries >

2022 , Vol. 48 >Issue 18: 292 - 299

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030206

Peanut allergenic protein and current methods of detection: A review

  • WANG Chengbin ,
  • HU Xiaofei ,
  • SUN Yaning ,
  • XING Yunrui ,
  • PANG Xinghao ,
  • WANG Lin ,
  • WU Jiabei ,
  • WANG Yao
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  • 1(College of Food and Bioengineering,Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Henan University of Science and Technology, Luoyang 471023, China)
    2(Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)

Received date: 2021-11-24

  Revised date: 2021-12-23

  Online published: 2022-10-17

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Abstract

Peanuts are one of the main oil crops and a valuable source of protein for consumers, but they can cause allergic reactions and even death in allergic individuals. So far, there is no exact treatment for allergic reactions, avoiding allergic food is the main way to ensure food safety for allergic patients. Thus, it is particularly important to strengthen the monitoring of peanut allergenic protein in food for food allergenic risk assessment and supervision. This review summarizes the detection methods of peanut allergenic protein from the protein and DNA levels, and the development trend of its detection methods is prospected, in order to provide reference for further research and application of peanut allergenic protein detection methods.

Key words： peanuts; allergenic protein; peanut allergy; detection method

Cite this article

WANG Chengbin , HU Xiaofei , SUN Yaning , XING Yunrui , PANG Xinghao , WANG Lin , WU Jiabei , WANG Yao . Peanut allergenic protein and current methods of detection: A review[J]. Food and Fermentation Industries, 2022 , 48(18) : 292 -299 . DOI: 10.13995/j.cnki.11-1802/ts.030206

References

[1] MUMTAZ S, ALI S, KHAN R, et al.Therapeutic role of garlic and vitamins C and E against toxicity induced by lead on various organs[J].Environmental Science and Pollution Research International, 2020, 27(9):8 953-8 964.
[2] AHMED M, ALZOUBI K H, KHABOUR O F.Vitamin E prevents the cognitive impairments in post-traumatic stress disorder rat model:Behavioral and molecular study[J].Psychopharmacology, 2020, 237(2):599-607.
[3] CHIZOBA EKEZIE F G, CHENG J H, SUN D W.Effects of nonthermal food processing technologies on food allergens:A review of recent research advances[J].Trends in Food Science & Technology, 2018, 74:12-25.
[4] LIEBERMAN J A, GUPTA R S, KNIBB R C, et al.The global burden of illness of peanut allergy:A comprehensive literature review[J].Allergy, 2021, 76(5):1 367-1 384.
[5] HOURIHANE J O, KILBURN S A, DEAN P, et al.Clinical characteristics of peanut allergy[J].Clinical and Experimental Allergy, 1997, 27(6):634-639.
[6] 黄玉霞, 梁金玲, WANG L, 等.食品中花生过敏原及其检测方法的研究进展[J].食品工业科技, 2018, 39(22):314-318;327.
HUANG Y X, LIANG J L, WANG L, et al.Research progress of peanut allergen and its detection methods[J].Science and Technology of Food Industry, 2018, 39(22):314-318;327.
[7] 赵兴华. 栽培种花生过敏原蛋白的鉴定与分子进化研究[D].福州:福建农林大学, 2020.
ZHAO X H.Identification and molecular evolution of allergen protein in cultivated peanut (Arachis hypogaea)[D].Fuzhou:Fujian Agriculture and Forestry University, 2020.
[8] IQBAL A, SHAH F, HAMAYUN M, et al.Allergens of Arachis hypogaea and the effect of processing on their detection by ELISA[J].Food & Nutrition Research, 2016, 60:28945.
[9] FILEP S, BLOCK D S, SMITH B R E, et al.Specific allergen profiles of peanut foods and diagnostic or therapeutic allergenic products[J].Journal of Allergy and Clinical Immunology, 2018, 141(2):626-631.e7.
[10] 王俊娟, 李欣芮, 陈成, 等.花生主要过敏原Ara h 1线性B细胞表位的预测及鉴定[J].食品科学, 2021, 42(17):106-112.
WANG J J, LI X R, CHEN C, et al.Prediction and identification of linear B cell epitope on the major peanut allergen Ara h 1[J].Food Science, 2021, 42(17):106-112.
[11] 田阳, 饶欢, 陶莎, 等.花生致敏蛋白Ara h 2提取纯化及免疫分析[J].粮食与油脂, 2016, 29(12):29-32.
TIAN Y, RAO H, TAO S, et al.Purification and immune identification of peanut allergen Ara h 2[J].Cereals & Oils, 2016, 29(12):29-32.
[12] WALCZYK N E, SMITH P M C, TOVEY E R, et al.Peanut protein extraction conditions strongly influence yield of allergens Ara h 1 and 2 and sensitivity of immunoassays[J].Food Chemistry, 2017, 221:335-344.
[13] POMÉS A, DAVIES J M, GADERMAIER G, et al.WHO/IUIS Allergen Nomenclature:Providing a common language[J].Molecular Immunology, 2018, 100:3-13.
[14] WANG Y, FU T J, HOWARD A, et al.Crystal structure of peanut (Arachis hypogaea) allergen Ara h 5[J].Journal of Agricultural and Food Chemistry, 2013, 61(7):1 573-1 578.
[15] 詹少德, 邱昌将, 朱盼, 等.花生过敏原蛋白Ara h 6基因克隆和原核表达[J].食品科学, 2016, 37(3):125-130.
ZHAN S D, QIU C J, ZHU P, et al.Gene cloning and prokaryotic expression of peanut allergen Ara h 6[J].Food Science, 2016, 37(3):125-130.
[16] APOSTOLOVIC D, MARSH J T, BAUMERT J, et al.Purification and initial characterization of Ara h 7, a peanut allergen from the 2S albumin protein family[J].Journal of Agricultural and Food Chemistry, 2021, 69(22):6 318-6 329.
[17] PANDEY A K, VARSHNEY R K, SUDINI H K, et al.An improved enzyme-linked immunosorbent assay (ELISA) based protocol using seeds for detection of five major peanut allergens Ara h 1, Ara h 2, Ara h 3, Ara h 6, and Ara h 8[J].Frontiers in Nutrition, 2019, 6:68.
[18] KRAUSE S, REESE G, RANDOW S, et al.Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population[J].Journal of Allergy and Clinical Immunology, 2009, 124(4):771-778.e5.
[19] PALLADINO C, BREITENEDER H.Peanut allergens[J].Molecular Immunology, 2018, 100:58-70.
[20] CABANOS C, URABE H, TANDANG-SILVAS M R, et al.Crystal structure of the major peanut allergen Ara h 1[J].Molecular Immunology, 2011, 49(1-2):115-123.
[21] MUELLER G A, GOSAVI R A, POMÉS A, et al.Ara h 2:Crystal structure and IgE binding distinguish two subpopulations of peanut allergic patients by epitope diversity[J].Allergy, 2011, 66(7):878-885.
[22] JIN T C, GUO F, CHEN Y W, et al.Crystal structure of Ara h 3, a major allergen in peanut[J].Molecular Immunology, 2009, 46(8-9):1 796-1 804.
[23] JOERIS T, MÜLLER-LUDA K, AGACE W W, et al.Diversity and functions of intestinal mononuclear phagocytes[J].Mucosal Immunology, 2017, 10(4):845-864.
[24] WAMBRE E, BAJZIK V, DELONG J H, et al.A phenotypically and functionally distinct human T H2 cell subpopulation is associated with allergic disorders[J].Science Translational Medicine, 2017, 9(401):eaam9171.
[25] KANAGARATHAM C, EL ANSARI Y S, LEWIS O L, et al.IgE and IgG antibodies as regulators of mast cell and basophil functions in food allergy[J].Frontiers in Immunology, 2020, 11:603050.
[26] 傅玲琳, 王彦波.食物过敏:从致敏机理到控制策略[J].食品科学, 2021, 42(19):1-19.
FU L L, WANG Y B.Food allergy:From sensitization mechanism to control strategy[J].Food Science, 2021, 42(19):1-19.
[27] XU J Y, YE Y L, JI J, et al.Advances on the rapid and multiplex detection methods of food allergens[J].Critical Reviews in Food Science and Nutrition, 2021:1-21.
[28] WU Z H, ZHANG Y, ZHAN S D, et al.Development of immunoaffinity chromatographic method for Ara h 2 isolation[J].Protein Expression and Purification, 2017, 131:85-90.
[29] 王耀, 刘胜男, 邢荣花, 等.双抗体夹心ELISA检测花生致敏蛋白Ara h 2的方法建立[J].食品工业, 2021, 42(3):290-293.
WANG Y, LIU S N, XING R H, et al.Establishment of double antibody sandwich ELISA for peanut allergenic protein Ara h 2[J].The Food Industry, 2021, 42(3):290-293.
[30] SAYERS R L, JOHNSON P E, MARSH, J T, et al.The effect of thermal processing on the behaviour of peanut allergen peptide targets used in multiple reaction monitoring mass spectrometry experiments[J].The Analyst, 2016, 141(13):4 130-4 141.
[31] 周红菲, 吴志华, 张英, 等.质谱法分析烘焙对花生过敏原Ara h 1潜在致敏性的影响[J].食品科学, 2021, 42(3):1-6.
ZHOU H F, WU Z H, ZHANG Y, et al.Effect of roasting on the potential allergenicity of peanut allergen Ara h 1 evaluated by mass spectrometry[J].Food Science, 2021, 42(3):1-6.
[32] 左树娜. 可视化SRCA检测食品中花生过敏原Ara h 1基因的研究[D].保定:河北农业大学, 2020.
ZUO S N.Study on the detection of peanut allergen protein Ara h 1 gene in food by visual saltatory rolling circle amplification[D].Baoding:Hebei Agricultural University, 2020.
[33] SANCHIZ A, SNCHEZ-ENCISO P, CUADRADO C, et al.Detection of peanut allergen by real-time PCR:Looking for a suitable detection marker as affected by processing[J].Foods (Basel, Switzerland), 2021, 10(6):1 421.
[34] QIAN C, WANG R, WU H, et al.Recent advances in emerging DNA-based methods for genetically modified organisms (GMOs) rapid detection[J].TrAC Trends in Analytical Chemistry, 2018, 109:19-31.
[35] 王烁, 孙晓东, 钮冰, 等.不同加工方式对花生致敏性的影响[J].食品科技, 2020, 45(4):49-55.
WANG S, SUN X D, NIU B, et al.Effect of different processing methods on the allergenicity of peanut[J].Food Science and Technology, 2020, 45(4):49-55.
[36] SHINMOTO H, MATSUO Y, NAGANAWA Y, et al.Epitope analysis of peanut allergen Ara h 1 with human monoclonal IgM antibody 92-2[J].Cytotechnology, 2010, 62(4):307-311.
[37] DAVOUDZADEH D, CHEN A, VALCOUR A, et al.Inter-laboratory reproducibility and inter-assay correlation of the Turbo-MPTM radioimmunoassay for quantitative measurement of allergen-specific immunoglobulin E[J].Journal of Allergy and Clinical Immunology, 2004, 113(2):S290.
[38] YAPO-CREZOIT C C A, ANANGA A, YAPO F, et al.Comparison of major peanut allergens Ara h 1, Ara h 2 and Ara h 3 between peanut cultivars from Côte d’Ivoire and the USA using SDS PAGE and Western Blots[J].IOSR Journal of Pharmacy and Biological Sciences, 2017, 12(1):31-36.
[39] 詹少德. 花生过敏原Ara h 2免疫亲和层析分离方法的建立[D].南昌:南昌大学, 2016.
ZHAN S D.Development of immunoaffinity chromatographic method for peanut allergen Ara h 2 isolation[D].Nanchang:Nanchang University, 2016.
[40] XI J, SHI Q Q.Development of an indirect competitive ELISA kit for the detection of soybean allergenic protein gly m bd 28K[J].Food Analytical Methods, 2016, 9(11):2 998-3 005.
[41] PENG J, SONG S S, LIU L Q, et al.Development of sandwich ELISA and immunochromatographic strip for the detection of peanut allergen Ara h 2[J].Food Analytical Methods, 2015, 8(10):2 605-2 611.
[42] MONTSERRAT M, SANZ D, JUAN T, et al.Detection of peanut (Arachis hypogaea) allergens in processed foods by immunoassay:Influence of selected target protein and ELISA format applied[J].Food Control, 2015, 54:300-307.
[43] JANSSENDUIJGHUIJSEN L M, WICHERS H J, VAN NORREN K, et al.Detection of peanut allergen in human blood after consumption of peanuts is skewed by endogenous immunoglobulins[J].Journal of Immunological Methods, 2017, 440:52-57.
[44] 陈献雄, 邬玉兰, 吉琼梅, 等.花生主要过敏原Ara h 1单克隆抗体的制备与应用[J].免疫学杂志, 2017, 33(1):68-72.
CHEN X X, WU Y L, JI Q M, et al.Preparation and application of monoclonal antibodies against allergen Ara h 1 from peanut[J].Immunological Journal, 2017, 33(1):68-72.
[45] PILOLLI R, DE ANGELIS E, MONACI L.Streamlining the analytical workflow for multiplex MS/MS allergen detection in processed foods[J].Food Chemistry, 2017, 221:1 747-1 753.
[46] DALY M, ANSARI P, HÄUBL G, et al.Assessing almond and peanut allergens using commercially available immunoanalytical kits and LC-MS/MS:A case study[J].Journal of AOAC INTERNATIONAL, 2019, 101(1):96-101.
[47] 洪宇伟, 陈启, 张京顺, 等.超高效液相色谱-电喷雾质谱法检测花生过敏原Ara h 2[J].食品安全质量检测学报, 2015,6(5):1 895-1 902.
HONG Y W, CHEN Q, ZHANG J S, et al.Identification and quantification of peanut allergen Ara h 2 using ultra-performance liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety & Quality, 2015, 6(5):1 895-1 902.
[48] JAMES D, SCHMIDT A M.Use of an intron region of a chloroplast tRNA gene (trnL) as a target for PCR identification of specific food crops including sources of potential allergens[J].Food Research International, 2004, 37(4):395-402.
[49] HOUHOULA D, KOUSSISSIS S, LOUGOVOIS V, et al.Detection of peanut allergen traces with a real time PCR assay - the challenge to protect food-allergic consumers[J].Journal of Food Research, 2017, 7(1):32.
[50] 陈家杰, 王海燕, 梁秋妮, 等.两种PCR方法检测食品中花生过敏原Ara h 1成分[J].食品研究与开发, 2011, 32(9):69-74.
CHEN J J, WANG H Y, LIANG Q N, et al.Development of two PCR assays to detect peanut allergen Ara h 1 DNA residue in food products[J].Food Research and Development, 2011, 32(9):69-74.
[51] 兰海鸥. 大豆、核桃、花生过敏原基因三重实时荧光检测方法的建立及应用[D].兰州:西北民族大学, 2020.
LAN H O.Establishment and application of triplex real-time fluorescence detection method for TaqMan probe of allergen gene in soybean, walnut and peanut[D].Lanzhou:Northwest University for Nationalities, 2020.
[52] 李颖超. 加工处理对花生致敏原的影响及其机理[D].无锡:江南大学, 2016.
LI Y C.Effect of processing on peanut allergens and its mechanisms[D].Wuxi:Jiangnan University, 2016.
[53] NOTOMI T, OKAYAMA H, MASUBUCHI H, et al.Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Research, 2000, 28(12):e63.
[54] SHEU S C, TSOU P C, LIEN Y Y, et al.Development of loop-mediated isothermal amplification (LAMP) assays for the rapid detection of allergic peanut in processed food[J].Food Chemistry, 2018, 257:67-74.
[55] YUAN D, KONG J L, LI X X, et al.Colorimetric LAMP microfluidic chip for detecting three allergens:Peanut, sesame and soybean[J].Scientific Reports, 2018, 8:8 682.
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