For efficient preparation of rare ginsenoside C-Mc1 and C-Mc from ginsenoside Rc, a ginsenoside-3-O-β-D-glucosidase from Terrabacter ginsenosidimutans Gsoil 308 2T was cloned and expressed in Escherichia coli C41(DE3). The molecular weight of the purified enzyme was 72.4 kDa with the optimum pH and temperature of 7.0 and 40℃. Its Km value was 6.25 mmol/L, Vmax was 19.6 mmol/(h·L). To lower the cost, preparation of ginsenoside C-Mc1 and C-Mc was carried out with the crude enzyme preparation. The results showed that when 20 g/L ginsenoside Rc was enzymatically catalyzed at 40 ℃ for 24 h, C-Mc1 and C-Mc monomers were obtained with a molar yield of 74.9% for C-Mc1, and 11.1% for C-Mc. When 5 g/L ginsenoside Rc was enzymatically catalyzed at 40 ℃ for 60 h, ginsenoside C-Mc was obtained with a molar yield of 93.2%. The purities of resultant C-Mc1 and C-Mc were both over 90% according to HPLC analysis. The results provide references for industrial production of C-Mc1 and C-Mc.
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