Effects of Nahlsgen and collagen hydrolysate on protection and collagen synthesis in human skin fibroblasts

  • MA Yongqing ,
  • YAN Jiangang ,
  • WEI Ying ,
  • XU Yaguang ,
  • ZHANG Ruixue ,
  • QIN Xiuyuan
Expand
  • 1(Beijing Engineering Research Center of Protein & Functional Peptide Corporation Limited, Beijing 100015, China)
    2(Perfect (China) Co.Ltd., Zhongshan 528451, China)

Received date: 2021-12-03

  Revised date: 2022-01-12

  Online published: 2022-11-01

Abstract

The effect of Nahlsgen and protein hydrolysate in human skin fibroblasts (HSF) was studied. In this investigation, HSF were irradiated with ultraviolet B (UVB) ray to establish the cell model of light injury. The methyl thiazolyl tetrazolium test (MTT test) was applied to evaluate the effect of Nahlsgen and collagen hydrolysate on cell viability. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay was conducted to reactive oxygen species (ROS) production. The effects of Nahlsgen and collagen hydrolysate on hydroxyproline content and the expression of collagen I and elastin of HSF were measured by qRT-PCR and Western Blot. The results indicated that Nahlsgen and collagen hydrolysate improved the cell viability and decreased the levels of ROS to protect HSF from UVB photodamage. Nahlsgen and collagen hydrolysate promoted the expression of collagen and elastin to delay skin aging.

Cite this article

MA Yongqing , YAN Jiangang , WEI Ying , XU Yaguang , ZHANG Ruixue , QIN Xiuyuan . Effects of Nahlsgen and collagen hydrolysate on protection and collagen synthesis in human skin fibroblasts[J]. Food and Fermentation Industries, 2022 , 48(19) : 86 -91 . DOI: 10.13995/j.cnki.11-1802/ts.030321

References

[1] 任静, 马良娟.Nrf2基因在紫外线氧化应激与光老化中的作用研究进展[J].实用皮肤病学杂志, 2018, 11(2):94-96;100.
REN J, MA L J.Research on the role of Nrf2 in photoaging[J].Journal of Practical Dermatology, 2018, 11(2):94-96;100
[2] LAN C C E, HUNG Y T, FANG A H, et al.Effects of irradiance on UVA-induced skin aging[J].Journal of Dermatological Science, 2019, 94(1):220-228.
[3] LIU X M, ZHANG R Z, SHI H X, et al.Protective effect of curcumin against ultraviolet A irradiation-induced photoaging in human dermal fibroblasts[J].Molecular Medicine Reports, 2018, 17(5):7 227-7 237.
[4] WU P Y, HUANG C C, CHU Y, et al.Alleviation of ultraviolet B-induced photodamage by Coffea arabica extract in human skin fibroblasts and hairless mouse skin[J].International Journal of Molecular Sciences, 2017, 18(4):782.
[5] 薛艳丽. 胶原蛋白与美容[J].中国实用医药, 2006, 1(9):84.
XUE Y L.Collagen and beauty[J].China Practical Medical, 2006, 1(9):84.
[6] 张浩玮, 胡竟一, 王岚.胶原蛋白在整形美容中的研究进展[J].科技视界, 2019(2):152-154.
ZHANG H W, HU J Y, WANG L.Research progress of collagen in cosmetic surgery[J].Science & Technology Vision, 2019(2):152-154.
[7] 贾建萍, 鲁健章, 周彦钢, 等.不同来源胶原肽的抗氧化活性及吸湿保湿性能[J].食品科学, 2010, 31(21):169-172.
JIA J P, LU J Z, ZHOU Y G, et al.Antioxidant activity, moisture-absorbing and moisture-retention properties of collagen peptides from different sources[J].Food Science, 2010, 31(21):169-172.
[8] 汪雨亭, 黄儒强, 王娟.鱼胶原蛋白酶解工艺及其活性肽抑制ACE酶的研究[J].食品工业科技, 2017, 38(17):17-23.
WANG Y T, HUANG R Q, WANG J.Study on enzymatic hydrolysis process of fish collagen and antihypertensive peptides of collagen[J].Science and Technology of Food Industry, 2017, 38(17):17-23.
[9] 李承威, 马淑霞, 王春敏, 等.胶原蛋白肽增强小鼠免疫力的实验研究[J].中国微生态学杂志, 2013, 25(4):387-389.
LI C W, MA S X, WANG C M, et al.Collagen peptide enhances immunity in mice[J].Chinese Journal of Microecology, 2013, 25(4):387-389.
[10] KANG M C, YUMNAM S, KIM S Y.Oral intake of collagen peptide attenuates ultraviolet B irradiation-induced skin dehydration in vivo by regulating hyaluronic acid synthesis[J].International Journal of Molecular Sciences, 2018, 19(11):3551.
[11] LIU Z H, LI Y Q, SONG H D, et al.Collagen peptides promote photoaging skin cell repair by activating the TGF-β/Smad pathway and depressing collagen degradation[J].Food & Function, 2019, 10(9):6 121-6 134.
[12] KAMIYAMA A, NAKAJIMA M, HAN L Y, et al.Phosphonate-based irreversible inhibitors of human γ-glutamyl transpeptidase (GGT).GGsTop is a non-toxic and highly selective inhibitor with critical electrostatic interaction with an active-site residue Lys562 for enhanced inhibitory activity[J].Bioorganic & Medicinal Chemistry, 2016, 24(21):5 340-5 352.
[13] WATANABE B, TABUCHI Y, WADA K, et al.Synthesis and evaluation of the inhibitory activity of the four stereoisomers of the potent and selective human γ-glutamyl transpeptidase inhibitor GGsTop[J].Bioorganic & Medicinal Chemistry Letters, 2017, 27(21):4 920-4 924.
[14] 刘文颖, 林峰, 金振涛, 等.玉米低聚肽的体外抗氧化作用[J].食品科学, 2011, 32(5):22-26.
LIU W Y, LIN F, JIN Z T, et al.Antioxidant effect of corn oligopeptides in vitro[J].Food Science, 2011, 32(5):22-26.
[15] 张杰, 甘华, 刘瑾.UVB诱导人皮肤成纤维细胞改变后β-catenin基因表达水平的研究[J].西部医学, 2019, 31(1):47-50.
ZHANG J, GAN H, LIU J.Study on the change of β-catenin in HSF with induced by UVB[J].Medical Journal of West China, 2019, 31(1):47-50.
[16] 张瑞雪, 方磊, 刘艳, 等.三种果蔬发酵液抗氧化和免疫调节功能研究[J].粮油食品科技, 2019, 27(2):39-44.
ZHANG R X, FANG L, LIU Y, et al.Research on antioxidant and immunity regulation function of three kinds of fruit and vegetable zymotic fluid[J].Science and Technology of Cereals, Oils and Foods, 2019, 27(2):39-44.
[17] 石宇, 李德如, 闫国富, 等.芦荟凝胶对人皮肤成纤维细胞增殖及胶原合成的影响[J].重庆医学, 2008, 37(12):1 271-1 273;1 264.
SHI Y, LI D R, YAN G F, et al.Effect of aloe gel on cell proliferation and synthesis of collage in cultured human skin fibroblast[J].Chongqing Medicine, 2008, 37(12):1 271-1273;1 264.
[18] DETERS A M, MEYER U, STINTZING F C.Time-dependent bioactivity of preparations from cactus pear (Opuntia ficus indica) and ice plant (Mesembryanthemum crystallinum) on human skin fibroblasts and keratinocytes[J].Journal of Ethnopharmacology, 2012, 142(2):438-444.
[19] SANTA-MARÍA C, REVILLA E, MIRAMONTES E, et al.Protection against free radicals (UVB irradiation) of a water-soluble enzymatic extract from rice bran.Study using human keratinocyte monolayer and reconstructed human epidermis[J].Food and Chemical Toxicology, 2010, 48(1):83-88.
[20] ZOROV D B, FILBURN C R, KLOTZ L O, et al.Reactive oxygen species (ROS)-induced ROS release:A new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes[J].The Journal of Experimental Medicine, 2000, 192(7):1 001-1 014.
[21] POLJŠAK B, DAHMANE R G, GODIĆ A.Intrinsic skin aging:The role of oxidative stress[J].Acta Dermatovenerologica Alpina, Pannonica, et Adriatica, 2012, 21(2):33-36.
[22] SANCHES SILVEIRA J E, MYAKI PEDROSO D M.UV light and skin aging[J].Reviews on Environmental Health, 2014, 29(3):243-254.
Outlines

/