Pimelic acid is an important dicarboxylic acid, which is broadly applied in medicine, polymer manufacturing and other industries. Currently, the biosynthesis of pimelic acid has the problems of low yield and few kinds of hosts, which limits its further industrial production and derivatives development. In order to increase the biosynthesis yield of pimelic acid, this study firstly used 48-well plate and shake flasks for primary screening and re-screening, respectively, obtaining a pimelic acid-producing Enterobacter hormaechei Bpa-3, of which the pimelic acid titer was 115.4 mg/L during shake flask fermentation. Subsequently, the fermentation conditions of strain Bpa-3 were optimized. It was found that when the carbon source was glycerol, the nitrogen source was tryptone, the amount of inoculum was 4% and adding 30 mmol/L KH2PO4, the titer of pimelic acid was the highest, which was 217.3 mg/L and1.89-fold than that before optimization, providing a new host for the biosynthesis of pimelic acid and laying foundations for its industrial development.
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