Food and Fermentation Industries >

2022 , Vol. 48 >Issue 22: 82 - 88

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030666

Preparation and antifungal activity of chitosan nanoparticle loaded with carvacrol

  • XING Chengyu ,
  • WANG Lu ,
  • CHEN Xingguang ,
  • LU Jian ,
  • WU Dianhui
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  • 1(National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing (Jiangnan University), Wuxi 214122, China)
    2(Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, School of Biotechnology, Wuxi 214122, China)
    3(Jiangsu Engineering Technology Research Center for Bioactive Products Processing, Wuxi 214122, China)

Received date: 2021-12-31

  Revised date: 2022-02-10

  Online published: 2022-12-20

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Abstract

Fusarium graminearum and its mycotoxins seriously endanger the quality and safety of grains. The development of new green antifungal agents is still one of the important ways to control grain mildew and ensure food safety. In this study, carvacrol-loaded chitosan nanoparticles were prepared by emulsion-ionic gelation. The results showed that the retention rate of carvacrol ranged from 35% to 60%, and the resulting nanoparticles displayed regular distribution and spherical shape, the size range of which were 40-100 nm. The stability of antifungal activity of carvacrol and carvacrol-chitosan nanoparticles against F. graminearum was investigated by accelerated storage. After 50 d of storage at 35 ℃, the mycelial inhibition against F. graminearum by carvacrol and carvacrol-chitosan nanoparticles decreased from 82.2% to 25.1% and 70.5% to 47.7%, respectively. It indicated that the preparation of nanoparticles improved the inhibition stability of carvacrol. The results of scanning electron microscopy showed that the carvacrol-loaded chitosan nanoparticles could significantly damage the cell membrane and cell wall of F. graminearum, thereby inhibiting its growth. This study provides a new idea to address the contamination of grain mold and fungal toxins in grain.

Key words： Fusarium graminearum; carvacrol; chitosan nanoparticles; retention rate; antifungal stability

Cite this article

XING Chengyu , WANG Lu , CHEN Xingguang , LU Jian , WU Dianhui . Preparation and antifungal activity of chitosan nanoparticle loaded with carvacrol[J]. Food and Fermentation Industries, 2022 , 48(22) : 82 -88 . DOI: 10.13995/j.cnki.11-1802/ts.030666

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