To find out the differences in physicochemical properties and biological activities of Akebia trifoliata pectin from different regions, the pectins in the peel of Akebia trifoliata from two regions (Kaili, Guizhou and Nanyang, Henan) were extracted using acid ex- traction and alcohol precipitation. The physicochemical properties, the ability to scavenge ·OH and DPPH free radicals, and the inhibi- tory effect on human lung cancer cells(A549)and human chronic myeloid leukemia cells (K562-2) were compared between these two extracted pectins. It was found that the pectin extracted by acid extraction and alcohol precipitation from both regions belonged to the cate- gory of high ester pectin, and its galacturonic acid content and esterification degree were higher than that of commercial pectin, which had a certain scavenging effect on ·OH and DPPH free radicals. At low concentrations (≤0.5 mg/mL), there was no significant difference in the ability of these two pectins to remove ·OH from the two regions. However, as the pectin concentration increased (>0.5 mg/mL), the scavenging capacity of Nanyang pectin in Henan province was significantly higher than that of Kaili pectin in Guizhou province. The results by MTT showed that the pectin from both producing areas had the inhibitory ability on the growth of cancer cells A549 and K562-2. The inhibitory rate of Kaili pectin on A549 and K562-2 was slightly higher than that of Nanyang pectin. This study reveals that the peel pectin of Akebia trifoliata has a better development prospect in medicine and health care and provides a certain theoretical basis for the development and utilization of Akebia trifoliata from different areas.
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