Food and Fermentation Industries >

2022 , Vol. 48 >Issue 22: 157 - 164

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031075

Differential analysis of functional properties of exopolysaccharides from five strains of Bifidobacterium

  • WANG Xiaonan ,
  • CHEN Shiyang ,
  • ZHANG Lijie ,
  • GAO Xinyang ,
  • CHEN Shuxing ,
  • RU Yuanpu
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(School of Food Science and Engineering, Zhengzhou University of Science and Technology, Zhengzhou 450064, China)
    3(Luohe Food Vocational College, Luohe 462333, China)
    4(National Engineering Center of Dairy for Maternal and Child Health, Beijing Engineering Research Center of Dairy, Beijing Technical Innovation Center of Human Milk Research, Beijing Sanyuan Foods Co.Ltd., Beijing 100163, China)

Received date: 2022-02-10

  Revised date: 2022-03-01

  Online published: 2022-12-20

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Abstract

The aim of this study is to analyze and compare the physical and chemical properties of exopolysaccharides from five strains of Bifidobacterium (Bifidobacterium pseudocatenulatum BP-1, Bifidobacterium longum BL-3, Bifidobacterium animalis BA-5, Bifidobacterium animalis BA-6, and Bifidobacterium infantis BI-38). The exopolysaccharides from five strains of Bifidobacterium were extracted by water extraction and alcohol precipitation, and their antioxidant activity, antibacterial properties, apparent morphology, functional group types and rheological properties were analyzed and compared. The results showed that the yield of BA-5 was(552.69±3.38) mg/L, which was significantly higher than that of the other strains(P<0.05);In vitro antioxidant tests results showed that the five exopolysac- charides exhibited strong scavenging ability on both DPPH radical and hydroxyl radical. Among these, the scavenging rate of BA-6 exopolysaccharides to superoxide anion radical reached(67.78±0.68)%, which was significantly higher than that of other exopolysacchar- ides. The results of the antibacterial test showed that all of the five exopolysaccharides had antibacterial activities against Escherichia coli, Staphylococcus aureus and Listeria monocytogenes, of which BA-6 exopolysaccharides had a highest bacteriostatic rate of (94.96±1.43)% against L. monocytogenes. There were differences among the apparent morphologies of the five exopolysaccharides under scanning electron microscope, and there were no differences among types of functional groups in exopolysaccharides with Fourier middle far transform infrared spectroscopy. The apparent viscosity of exopolysaccharides solution decreased with the increase of shear rate, typical shear thinning non-Newtonian fluid characteristics was certified, and BA-6 exopolysaccharides had higher initial apparent viscosity than the others. Differential scanning calorimetry results indicated that all five exopolysaccharides showed higher thermal stability. These results showed that the functional properties of Bifidobacterium exopolysaccharides from different sources and species were different, and those of B. animalis BA-6 was better and had higher potential value for application.

Key words： Bifidobacterium; exopolysaccharides; antioxidant capacity; antibacterial properties; differential analysis

Cite this article

WANG Xiaonan , CHEN Shiyang , ZHANG Lijie , GAO Xinyang , CHEN Shuxing , RU Yuanpu . Differential analysis of functional properties of exopolysaccharides from five strains of Bifidobacterium[J]. Food and Fermentation Industries, 2022 , 48(22) : 157 -164 . DOI: 10.13995/j.cnki.11-1802/ts.031075

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