Food and Fermentation Industries >

2022 , Vol. 48 >Issue 22: 213 - 219

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029775

Separation and purification of antimicrobial peptides from lactoferrin by DA201-C macroporous adsorbent resin

  • ZHANG Lin ,
  • CAI Yinchuan ,
  • HAO Gang ,
  • HU Ting ,
  • JIANG Siyu
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  • 1(College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China)
    2(The Research Institute of Industrial Economy of Aba, Wenchuan 623000, China)

Received date: 2021-10-22

  Revised date: 2021-11-12

  Online published: 2022-12-20

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Abstract

In this study, the separation and purification conditions of lactoferrin antimicrobial peptide solution (molecular weight<3 000) treated by ultrafiltration with DA201-C macroporous resin were optimized by using adsorption capacity, resin dynamic adsorption and gradient elution of different ethanol concentrations as indexes. Static adsorption results showed that the adsorption capacity was 479.7 mg/g and the adsorption rate was 95.94% after 12 h of resin adsorption. Static desorption of saturated resin with different concentrations of ethanol showed that the desorption rate of 75% (volume fraction) ethanol reached 95.35%. The optimal adsorption conditions were determined as follows: NaCl concentration 0.6 mol/L, loading flow rate 1.5 mL/min and loading mass concentration 20 mg/mL. The gradient elution of lactoferrin antimicrobial peptides with different concentrations of ethanol was used as the index. The results showed that 75% ethanol eluted fraction had the strongest antibacterial activity against E. coli and Staphylococcus aureus, and the antibacterial rates of E. coli and Staphylococcus aureus reached 80.95% and 74.42%, respectively, which increased about 12.2% compared with before purification. Amino acid analysis of different elution components showed that the greater hydrophobicity and positive charge of the antimicrobial peptides, the stronger antibacterial activity.

Key words： lactoferrin; antimicrobial peptides; macroporous adsorption resin; ultrafiltration; separation and purification

Cite this article

ZHANG Lin , CAI Yinchuan , HAO Gang , HU Ting , JIANG Siyu . Separation and purification of antimicrobial peptides from lactoferrin by DA201-C macroporous adsorbent resin[J]. Food and Fermentation Industries, 2022 , 48(22) : 213 -219 . DOI: 10.13995/j.cnki.11-1802/ts.029775

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