Food and Fermentation Industries >

2022 , Vol. 48 >Issue 22: 255 - 262

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031255

Simultaneous detection of Vibrio parahaemolyticus and Staphylococcus aureus by real-time LAMP    

  • LIU Dan ,
  • XIE Jialing ,
  • ZHANG Mengyu ,
  • CHEN Song ,
  • LI Zi ,
  • ZHONG Qingping
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  • (Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China)

Received date: 2022-02-23

  Revised date: 2022-03-10

  Online published: 2022-12-20

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Abstract

In this study, in order to establish a real-time loop-mediated isothermal amplification (RT-LAMP) assay that can detect Vibrio parahaemolyticus and Staphylococcus aureus simultaneously, primers were designed for the specific genes blaCARB-17 and nuc of V. parahaemolyticus and S. aureus, and the reaction conditions of the assay were optimized. The specificity, sensitivity and applicability of this method were also studied. The results confirmed the high specificity and sensitivity of the method. The limits of detection for V. parahaemolyticus and S. aureus were 3.62×102 and 1.45×104 copies/mL, respectively. The sensitivities were 10 times of those of ordinary PCR methods. The two foodborne pathogens could be detected simultaneously in one LAMP reaction, which not only shortens the detection time, but also improves the detection efficiency, and provides a reference for the study of multiple LAMP detection of foodborne pathogens.

Key words： foodborne pathogens; Vibrio parahaemolyticus; Staphylococcus aureus; real-time LAMP; rapid detection

Cite this article

LIU Dan , XIE Jialing , ZHANG Mengyu , CHEN Song , LI Zi , ZHONG Qingping . Simultaneous detection of Vibrio parahaemolyticus and Staphylococcus aureus by real-time LAMP    [J]. Food and Fermentation Industries, 2022 , 48(22) : 255 -262 . DOI: 10.13995/j.cnki.11-1802/ts.031255

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