Lycopene is a terpenoid compound with high commercial value, which has good antioxidant properties and has a preventive effect on cardiovascular diseases. However, as an antioxidant, lycopene has certain toxicity to cells. According to studies, increasing the intracellular lipid content can effectively alleviate the toxic stress. In this manuscript, we overexpressed the key genes dga1 (acyl-CoA: diacylglycerol acyltransferase) and pah1 (phosphatidic acid phosphatase) of the triacylglycerol pathway (TAG pathway), and knock-in the ldp1 gene (lipid droplet attachment protein) as well, which improved the accumulation of lycopene in the cell. Sufficient precursor is provided by knocking out the ypl062w locus and simultaneously expressing the acs1L641P gene from Salmonella enterica heterologously, for both the lycopene synthesis and the TAG accumulation. pap1 (polynucleotide adenylyltransferase) was overexpressed to meet the transcription requirements of multiple gene overexpression. A cell factory suitable for lycopene accumulation was constructed, in which the unit yield of lycopene reached 109.26 mg/g DCW. A high-yielding lycopene recombinant strain was obtained through metabolic transformation, which laid the foundation for subsequent fermentation optimization.
LI Fangdi
,
LI Youran
,
ZHANG Liang
,
DING Zhongyang
,
GU Zhenghua
,
SHI Guiyang
,
XU Sha
. Metabolic engineering of Saccharomyces cerevisiae for lycopene production[J]. Food and Fermentation Industries, 2022
, 48(23)
: 25
-33
.
DOI: 10.13995/j.cnki.11-1802/ts.030710
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