Food and Fermentation Industries >

2022 , Vol. 48 >Issue 23: 132 - 137

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028676

Effects of fermentation crude extracts of two Lactobacillus plantarum strains on the growth of Lactobacillus brevis

  • HU Huilin ,
  • DAI Jing ,
  • LIU Caiqin ,
  • LU Yin ,
  • XIE Guangfa ,
  • MAO Qingzhong ,
  • ZHENG Lianbao
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  • 1(College of Biology and Environment Engineering, Zhejiang Shuren University, Hangzhou 310015, China)
    2(Huijishan Shaoxing Liquor Co., Ltd, Shaoxing 312000, China)
    3(DeQing Food and Drug Inspection and Testing Center, Huzhou 313200, China)

Received date: 2021-07-29

  Revised date: 2021-12-20

  Online published: 2023-01-05

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Abstract

The experiment discussed the effect of fermentation crude extracts of two Lactobacillus plantarum strains (R1 and R2) on the growth of Lactobacillus brevis. The growth, changes of intracellular and extracellular inorganic phosphorus and ATP of L. brevis cell were investigated. The results showed that the antibacterial activities of fermentation crude extracts of L. plantarum R1 and R2 against L. brevis were 160 AU/mL and 320 AU/mL, respectively. The fermentation crude extracts of L. plantarum R1 and R2 acted on L. brevis for 120 min, the L. brevis cell lysis rates reached 18.9% and 20.1%, respectively. Action with fermentation crude extract of L. plantarum R1 for 90 min, the intracellular inorganic phosphorus and ATP concentration of L. brevis declined by 1.135 mmol/L and 16.36 μmol/g Hb, respectively. Accordingly, the extracellular inorganic phosphorus and ATP concentrations of L. brevis increased by 0.894 mmol/L and 20.23 μmol/g Hb, respectively. Action with fermentation crude extract of L. plantarum R2 for 90 min, the intracellular inorganic phosphorus and ATP concentration of L. brevis decreased by 1.601 mmol/L and 21.56 μmol/g Hb, respectively. Accordingly, the extracellular inorganic phosphorus and ATP concentrations of L. brevis increased by 1.330 mmol/L and 23.40 μmol/g Hb, respectively. The results showed that the fermentation crude extracts of L. plantarum (R1 and R2) could inhibit the growth and destroy cells of L. brevis.

Key words： Lactobacillus plantarum; Lactobacillus brevis; fermentation crude extract; cell lysis rate; inorganic phosphorus concentration; ATP concentration

Cite this article

HU Huilin , DAI Jing , LIU Caiqin , LU Yin , XIE Guangfa , MAO Qingzhong , ZHENG Lianbao . Effects of fermentation crude extracts of two Lactobacillus plantarum strains on the growth of Lactobacillus brevis[J]. Food and Fermentation Industries, 2022 , 48(23) : 132 -137 . DOI: 10.13995/j.cnki.11-1802/ts.028676

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